色谱

色谱 ›› 2015, Vol. 33 ›› Issue (7): 777-782.DOI: 10.3724/SP.J.1123.2015.01043

• 技术与应用 • 上一篇    

液相色谱法分析烟叶酸性洗涤纤维素的碱性氧化铜降解产物

郝卫强1,2, 王磊君1, 吴顺2, 岳邦毅2, 陈强2, 张培培2   

  1. 1. 上海烟草集团有限责任公司, 烟草行业卷烟烟气重点实验室, 上海 200082;
    2. 常州南京大学高新技术研究院, 江苏 常州 213164
  • 收稿日期:2015-01-29 出版日期:2015-07-08 发布日期:2015-07-27
  • 通讯作者: 郝卫强
  • 基金资助:

    烟草行业卷烟烟气重点实验室开放研究基金项目(SZBCW201300555);江苏省自然科学基金项目(BK20141163).

Analysis of alkaline CuO degradation products of acid detergent fiber from tobacco leaves by using liquid chromatography

HAO Weiqiang1,2, WANG Leijun1, WU Shun2, YUE Bangyi2, CHEN Qiang2, ZHANG Peipei2   

  1. 1. Key Laboratory of Cigarette Smoke Research, Shanghai Tobacco Group Co., Ltd., China National Tobacco Corporation (CNTC), Shanghai 200082, China;
    2. Changzhou High-Tech Research Institute of Nanjing University, Changzhou 213164, China
  • Received:2015-01-29 Online:2015-07-08 Published:2015-07-27

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摘要：

应用石油醚-乙醇(6 : 4, v/v)、30 g/L十二烷基硫酸钠溶液以及含20 g/L十六烷基三甲基溴化铵的0.5 mol/L硫酸溶液依次处理烟叶样品,制备得到烟叶酸性洗涤纤维素(ADF)。采用碱性氧化铜氧化降解方法制备得到ADF降解产物。以Ultimate XB C18色谱柱为固定相,甲醇和水为流动相,梯度洗脱,在柱温35 ℃、流速0.8 mL/min、检测波长280 nm和320 nm条件下,对6种烟叶样品的ADF降解产物进行了液相色谱分离,得到4个特征色谱峰。以这4个特征色谱峰为考察对象,可研究不同烟叶木质素组成的差异及其与烟叶吸味品质的关系。

关键词: 碱性氧化铜降解, 木质素, 酸性洗涤纤维素, 烟叶, 液相色谱

Abstract:

The acid detergent fiber (ADF) from tobacco leaves was obtained by treating the sample with petroleum ether-ethanol (6 : 4, v/v), 30 g/L sodium dodecylsulfate and 0.5 mol/L sulphuric acid containing 20 g/L hexadecyl trimethyl ammonium bromide successively. The ADF was degraded by the alkaline CuO oxidation procedure. In this work, six samples of ADF degradation products from tobacco leaves were prepared. The samples were analyzed by using gradient liquid chromatography (LC) where an Ultimate XB C18 column was used as stationary phase, with a mixture of methanol and water as mobile phase, at a column temperature of 35 ℃ and a flow rate of 0.8 mL/min. Dual wavelengths of 280 nm and 320 nm were chosen for the detection. It was found that there were four characteristic peaks for the ADF degradation products. By taking these peaks as research object, the optimum time for the degradation was found to be 5 h and the sample solution could be kept stable within 7 days. The established method may provide a new approach for the studies of the differences between lignin composition in different tobacco leaves and the relationship between lignin content and the smoking quality of tobacco leaves.

Key words: acid detergent fiber (ADF), alkaline CuO degradation, lignin, liquid chromatography (LC), tobacco leaves

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