色谱 ›› 2015, Vol. 33 ›› Issue (12): 1226-1233.DOI: 10.3724/SP.J.1123.2015.09010

• 专论与综述 • 上一篇    下一篇

基于电迁移的蛋白质制备技术和方法新进展

郝斐然, 付斌, 张养军, 钱小红   

  1. 军事医学科学院放射与辐射医学研究所, 北京蛋白质组研究中心, 蛋白质组学国家重点实验室, 北京 102206
  • 收稿日期:2015-09-14 出版日期:2015-12-08 发布日期:2012-06-21
  • 通讯作者: 张养军, 钱小红
  • 基金资助:

    国家重大科学计划项目(2012CB910603);国家高技术研究发展规划项目(2012AA020202);国家重大科学仪器设备开发专项项目(2012YQ12004407,2011YQ030139,2011YQ06008408,2013YQ14040506);国家自然科学基金项目(21275519,20735005,31100591).

Recent advances in preparative electromigration techniques for proteins

HAO Feiran, FU Bin, ZHANG Yangjun, QIAN Xiaohong   

  1. State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China
  • Received:2015-09-14 Online:2015-12-08 Published:2012-06-21

摘要:

基于电迁移的蛋白质制备技术是对一类分离和制备技术的统称,其特征是在电场的作用下对目标物质进行分离和纯化制备,这种技术在生物大分子和蛋白质组的研究中应用广泛。基于电迁移的制备技术主要包括制备型电泳、制备型电色谱、制备型等电聚焦和自由流电泳等。本文对每种制备型电迁移装置的设计、特点和基于该种装置的各种应用方法的优缺点进行了详细阐述,并列举了一些实例。另外,微量级制备型电泳因分离度高、回收率高以及高效快速的优点,在微量级生物样本分析中发挥着日益重要的作用,近年来备受关注,本文也着重关注了这方面的进展,并对基于电迁移的制备技术做了展望。

关键词: 蛋白质, 电迁移制备技术, 微量级制备型电泳, 制备型等电聚焦, 制备型电色谱, 制备型电泳, 自由流电泳, 综述

Abstract:

Preparative electromigration techniques are a category of separation and preparation techniques based on differential electromigration principle. These techniques are widely applied in biomacromolecule and proteome researches. Related techniques mainly include preparative electrophoresis, preparative electrochromatography, preparative isoelectric focusing and free flow electrophoresis and so on. We review each kind of the preparative electromigration techniques on its apparatus designs, applications, advantages and disadvantages in detail. Microscale preparative electrophoresis is a hot spot in recent years. Its high resolution, high recovery and high efficiency make it a more prominent role in the biological sample analysis. In this review, we focus on some advances in microscale preparative electrophoresis and also give an outlook on preparative electromigration techniques in the future.

Key words: free flow electrophoresis, microscale preparative electrophoresis, preparative electrochromatography, preparative electromigration technique, preparative electrophoresis, preparative isoelectric focusing, proteins, review

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