色谱 ›› 2018, Vol. 36 ›› Issue (11): 1088-1098.DOI: 10.3724/SP.J.1123.2018.04020

• 研究论文 • 上一篇    下一篇

基于鞘脂组学技术探究布洛芬对斑马鱼脑组织的立体选择性影响

宋月1,2,3, 张茜宁1,2, 张崴1,2, 钱永忠1,2, 邱静1,2   

  1. 1. 中国农业科学院农业质量标准与检测技术研究所, 北京 100081;
    2. 农业部农产品质量安全重点实验室, 北京 100081;
    3. 山东莱博生物科技有限公司, 山东 济南 250000
  • 收稿日期:2018-04-17 出版日期:2018-11-08 发布日期:2014-09-30
  • 通讯作者: 邱静.Tel:(010)82106551,E-mail:qiujing@caas.cn.
  • 基金资助:

    国家自然科学基金项目(21477161);中央级公益性科研院所基本科研业务费专项(Y2017JC46).

Stereoselective effects of ibuprofen on adult zebrafish brain tissues based on sphingolipidomics

SONG Yue1,2,3, ZHANG Xining1,2, ZHANG Wei1,2, QIAN Yongzhong1,2, QIU Jing1,2   

  1. 1. Institute of Quality Standard and Testing Technology for Agro-Products of Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China;
    3. Shandong LaiBo Biotechnology Co. Ltd., Jinan 250000, China
  • Received:2018-04-17 Online:2018-11-08 Published:2014-09-30
  • Supported by:

    National Natural Science Foundation of China (No. 21477161); the Central Public-Interest Scientific Institution Basal Research Fund (No. Y2017JC46).

摘要:

以斑马鱼成鱼为研究对象,借助于鞘脂组学技术,探究了一种新型手性环境污染物布洛芬对斑马鱼脑组织中鞘脂含量的影响。建立了超高效液相色谱-三重四极杆-线性离子阱复合质谱检测斑马鱼成鱼脑组织中46种鞘脂的定量方法。借助于该方法对经布洛芬外消旋体及对映体暴露后的斑马鱼脑组织中的46种鞘脂进行了定量分析,通过考察主成分分析(PCA)、正交偏最小二乘法分析(OPLS-DA)及显著性差异分析(P)筛选出差异代谢物。同时,结合组间差异分析的结果可知,环境水平的布洛芬会导致斑马鱼脑组织中鞘脂代谢发生紊乱,且布洛芬的对映体之间存在立体选择性毒性。

关键词: 斑马鱼, 布洛芬, 超高效液相色谱-串联质谱, 对映体, 鞘脂, 脂质组学

Abstract:

The aim of this study was to investigate the stereoselective effects of a popular pharmaceutical and personal care product component ibuprofen (IBU) on adult zebrafish using sphingolipidomics. Sphingolipidomics is an effective method developed to determine sphingolipids present in biological samples using ultra-high performance liquid chromatography-triple quadrupole linear ion trap mass spectrometry. Using this method, 46 sphingolipids in adult zebrafish brain samples were quantified after rac-/R-(-)-/S-(+)-IBU exposure, and the biomarkers were selected using chemometric approaches, including principal component analysis and orthogonal partial least squares discrimination analysis. Besides, combined with the results of the Duncan post hoc test and Dunnett post hoc comparison, sphingolipidomic perturbations were found to be induced after exposure to ibuprofen at environmental concentrations, and enantiomers of ibuprofen had stereoselective effects on zebrafish. This study may provide a new insight on the influence of ibuprofen on aquatic organisms.

Key words: enantiomers, ibuprofen, lipidomics, sphingolipid, ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), zebrafish

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