色谱

色谱 ›› 2018, Vol. 36 ›› Issue (11): 1189-1193.DOI: 10.3724/SP.J.1123.2018.06027

• 技术与应用 • 上一篇    

离子色谱-抑制型电导检测牛肝菌中胆碱、腐胺和尸胺

林立1, 李仁勇2, 王琳琳1, 邱云1   

  1. 1. 国家食品质量安全监督检验中心, 北京 100094;
    2. 中科谱研(北京)科技有限公司, 北京 100027
  • 收稿日期:2018-06-27 出版日期:2018-11-08 发布日期:2014-09-30
  • 通讯作者: 林立.E-mail:linli77422@aliyun.com.
  • 基金资助:

    2017-食品安全国家标准制定、修订项目（Spaq-2017-060）.

Determination of choline, putrescine and cadaverine in boletus by ion chromatography with suppressed conductivity detection

LIN Li1, LI Renyong2, WANG Linlin1, QIU Yun1   

  1. 1. China National Food and Safety Supervision and Inspection Center, Beijing 100094, China;
    2. Zhongke PUYAN(Beijing) Science and Technology Co., Ltd., Beijing 100027, China
  • Received:2018-06-27 Online:2018-11-08 Published:2014-09-30
  • Supported by:

    2017 National Standards for Food Safety Drawing and Revising Program (No. Spaq-2017-060).

PDF

95

摘要：

建立离子色谱-抑制型电导检测牛肝菌中胆碱、腐胺和尸胺的方法。牛肝菌干片样品经7 mmol/L甲烷磺酸溶液提取，过滤膜，经反相固相萃取柱净化后进样分析。胆碱、腐胺、尸胺与样品中共存离子在IonPac CS17（250 mm×4 mm）阳离子交换色谱柱上可实现较好分离。以7 mmol/L甲烷磺酸为淋洗液等度淋洗，25 min可完成一次样品测定。胆碱、腐胺和尸胺的检出限（S/N=3）分别为0.002、0.002和0.003 mg/L，具有较宽的线性范围（0.01~10 mg/L），样品加标回收率为91.6%~100.2%。该方法简便、选择性好、灵敏度高，适用于牛肝菌中胆碱、腐胺和尸胺的检测分析。

关键词: 胆碱, 腐胺, 离子色谱, 牛肝菌, 尸胺

Abstract:

A chromatographic method was developed for the determination of choline, putrescine and cadaverine in boletus by cation exchange chromatography with suppressed conductivity detection. Samples were extracted with 7 mmol/L methanesulfonic acid solution, then filtered through a membrane, and purified by a reversed phase solid phase extraction column. Choline, putrescine, cadaverine and coexisting ions in the samples were separated well on the IonPac CS17 cation exchange column (250 mm×4 mm). The mobile phase was 7 mmol/L methanesulfonic acid solution. All analytes could be eluted from the column in 25 min. The limits of detection (S/N=3) of choline, putrescine and cadaverine were 0.002, 0.002 and 0.003 mg/L, respectively. Moreover, they all had wide linearity ranges (0.01-10 mg/L) and satisfactory recoveries (91.6%-100.2%). This method is suitable for the determination of choline, putrescine and cadaverine in boletus, and has good separation performance, high selectivity and sensitivity.

Key words: boletus, cadaverine, choline, ion chromatography (IC), putrescine

中图分类号: