色谱

色谱 ›› 2018, Vol. 36 ›› Issue (12): 1297-1302.DOI: 10.3724/SP.J.1123.2018.08029

• 研究论文 • 上一篇    下一篇

离子色谱-积分脉冲安培法测定肉类产品中鹅肌肽、高肌肽及肌肽

朱作艺1,2, 张玉1, 王君虹1, 李雪1, 王伟1,2, 杨华1, 朱岩3   

  1. 1. 浙江省农业科学院农产品质量标准研究所, 浙江 杭州 310021;
    2. 农业农村部创意农业重点实验室, 浙江 杭州 310021;
    3. 浙江大学化学系, 浙江 杭州 310028
  • 收稿日期:2018-08-21 出版日期:2018-12-08 发布日期:2014-11-06
  • 通讯作者: 王伟,Tel:(0571)85384815,E-mail:wangwei5228345@126.com
  • 基金资助:

    国家现代农业产业技术体系之水禽产业技术体系（CARS-42-27）；浙江省重点研发计划项目（2017C02G4010648）.

Determination of anserine, homocarnosine and carnosine in meat products by ion chromatography with integrated pulsed amperometric detection

ZHU Zuoyi1,2, ZHANG Yu1, WANG Junhong1, LI Xue1, WANG Wei1,2, YANG Hua1, ZHU Yan3   

  1. 1. Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China;
    2. Key Laboratory of Creative Agriculture, Ministry of Agriculture and Rural Affairs, Hangzhou 310021, China;
    3. Department of Chemistry, Zhejiang University, Hangzhou 310028, China
  • Received:2018-08-21 Online:2018-12-08 Published:2014-11-06
  • Supported by:

    Waterfowl Industry Technology System of the National Modern Agricultural Industry Technology System (No. CARS-42-27); Zhejiang Provincial Key Research and Development Project (No. 2017C02G4010648).

PDF

132

摘要：

建立了一种离子色谱-积分脉冲安培（IC-IPAD）同时测定肉类样品中鹅肌肽、高肌肽及肌肽的分析方法。方法采用高效阴离子交换色谱柱AminoPac PA10（250 mm×2 mm）分离，以100 mmol/L NaOH为淋洗液，流速为0.2 mL/min，柱温为30℃。结果表明，3种目标化合物在15 min内可实现完全分离，且17种氨基酸对3种目标化合物不存在干扰。在最佳色谱条件下，鹅肌肽、高肌肽及肌肽在0.05~5.0 mg/L范围内呈良好的线性关系，线性相关系数（r）>0.99。3种目标化合物的检出限和定量限分别为8.9~22.1 μg/L和29.6~73.6 μg/L。对鸭胸及鹅胸样品进行分析，加标回收率为92.4%~104.5%。该方法简单方便，无需衍生化，灵敏度高，可用于肉类产品中相关营养成分的测定。

关键词: 鹅肌肽, 高肌肽, 肌肽, 积分脉冲安培, 离子色谱

Abstract:

A method was developed for the simultaneous determination of anserine, homocarnosine and carnosine in meat samples using ion chromatography (IC) with integrated pulsed amperometric detection (IPAD). The samples were separated on a high performance anion exchange AminoPac PA10 column (250 mm×2 mm) using 100 mmol/L NaOH as the eluent. The flow rate was kept at 0.2 mL/min and the column temperature was set at 30℃. The three target compounds were separated within 15 min, and there were no interferences from 17 tested amino acids in their determination. Under the optimal chromatographic conditions, anserine, homocarnosine and carnosine showed good linearity in the range of 0.05-5 mg/L with correlation coefficients (r) greater than 0.99. The limits of detection (LODs) and limits of quantification (LOQs) were in the range of 8.9-22.1 μg/L and 29.6-73.6 μg/L, respectively. The proposed method was applied to the analysis of duck breast and goose breast meat samples. The average spiked recoveries ranged from 92.4% to 104.5%. This simple and sensitive method can be applied to the determination of related nutrients in meat products.

Key words: anserine, carnosine, homocarnosine, integrated pulsed amperometric detection (IPAD), ion chromatography (IC)

中图分类号: