色谱

色谱 ›› 2019, Vol. 37 ›› Issue (6): 644-648.DOI: 10.3724/SP.J.1123.2018.12029

• 研究论文 • 上一篇    下一篇

中空纤维膜液相微萃取-超高效液相色谱测定蒙药毛勒日-达布斯-4汤中两种生物碱

宝贵荣1,2, 孟和3, 李优鑫2, 包建民2   

  1. 1. 呼和浩特职业学院生物化学工程学院, 内蒙古 呼和浩特 010051;
    2. 天津大学药物科学与技术学院, 天津 300072;
    3. 内蒙古大学化学化工学院, 内蒙古 呼和浩特 010021
  • 收稿日期:2018-12-20 出版日期:2019-06-08 发布日期:2015-04-17
  • 通讯作者: 包建民,Tel:(022)27892820,E-mail:jbao007@gmail.com.
  • 基金资助:

    内蒙古自然科学基金项目（2018MS02007）.

Determination of two alkaloids in Mongolian medicine Mauleri-Dabusi-4 soup by hollow fiber liquid phase micro-extraction coupled with ultra-high performance liquid chromatography

BAO Guirong1,2, MENG He3, LI Youxin2, BAO James Jianmin2   

  1. 1. Department of Biochemistry Engineering, Hohhot Vocational College, Hohhot 010051, China;
    2. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China;
    3. College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, China
  • Received:2018-12-20 Online:2019-06-08 Published:2015-04-17
  • Supported by:

    Inner Mongolia Natural Science Foundation (No. 2018MS02007).

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摘要：

建立了将中空纤维膜液相微萃取（HF-LPME）技术与超高效液相色谱（UPLC）技术联用检测蒙药毛勒日-达布斯-4汤中2种生物碱（胡椒碱和荜茇宁）的分析方法。通过考察该HF-LPME方法的影响参数，优化了萃取实验条件。HF-LPME优化条件如下：空隙率大于50%的偏氟乙烯中空纤维膜，萃取溶剂为正辛醇，氯化钠质量浓度为10 g/L，室温振荡，振荡速度为173 r/min，萃取时间为128 min。结果表明：该HF-LPME-UPLC方法对胡椒碱和荜菝宁的检出限（LOD）分别为2.2和2.5 μg/L，相对标准偏差不大于7.8%（n=5）。胡椒碱和荜菝宁分别在100~8500 和8.3~5000 μg/L范围内具有良好的线性关系，胡椒碱和荜茇宁的富集倍数分别为59和65。该方法简便、快速、准确、环保，适用于蒙药中胡椒碱和荜菝宁含量的测定。

关键词: 超高效液相色谱, 毛勒日-达布斯-4, 蒙药, 生物碱, 中空纤维膜液相微萃取

Abstract:

In this study, a method combining hollow fiber liquid phase micro-extraction (HF-LPME) and ultra-high performance liquid chromatography (UPLC) was developed for the determination of two alkaloids (piperine and piperlonguminine) in Mongolian medicine Mauleri-Dabusi-4 Soup. The parameters affecting the micro-extraction efficiency were evaluated and optimized. The optimum conditions were as follows:polyvinylidence fluoride HF (54% pore size); 10 g/L NaCl; 30 μ L octanol as extraction solvent; 173 r/min stirring rate of extraction; and 128 min extraction time. The extracted drug was detected by UPLC. The calibration curves obtained good linear relationship in the concentration ranges of 100-8500 and 8.3-5000 μg/L for piperine and piperlonguminine, respectively. The enrichment factors of the method for piperine and piperlonguminine were achieved to be 59 and 65. The limits of detection were 2.2 μg/L for piperine and 2.5 μg/L for piperlonguminine. The method was successfully applied for the determination of alkaloids in Mongolian medicine Mauleri-Dabusi-4.

Key words: alkaloids, hollow fiber liquid phase micro-extraction (HF-LPME), Mauleri-Dabusi-4, Mongolian medicine, ultra-high performance liquid chromatography (UPLC)

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