色谱

色谱 ›› 2019, Vol. 37 ›› Issue (10): 1105-1111.DOI: 10.3724/SP.J.1123.2019.03024

• 研究论文 • 上一篇    下一篇

超高效液相色谱-串联质谱法快速同时测定生姜中10种营养成分

张文焕, 刘平香, 邱静, 贾琪, 钱永忠   

  1. 中国农业科学院农业质量标准与检测技术研究所, 农业农村部农产品质量安全重点实验室, 北京 100081
  • 收稿日期:2019-03-20 出版日期:2019-10-08 发布日期:2020-12-11
  • 通讯作者: 钱永忠,Tel:(010)82106298,E-mail:qianyongzhong@caas.cn;邱静,Tel:(010)82106551,E-mail:qiujing@caas.cn.
  • 基金资助:
    国家特色蔬菜产业技术体系岗位专家任务（CARS-24-E-04）.

Rapid simultaneous determination of 10 nutrients in ginger by ultra-high performance liquid chromatography-tandem mass spectrometry

ZHANG Wenhuan, LIU Pingxiang, QIU Jing, JIA Qi, QIAN Yongzhong   

  1. Institute of Quality Standard and Testing Technology for Agro-Products of Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture and Rural Affairs, Beijing 100081, China
  • Received:2019-03-20 Online:2019-10-08 Published:2020-12-11
  • Supported by:
    National Specialty Vegetable Industry Technology System Post Expert Task (No. CARS-24-E-04).

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摘要： 建立了超高效液相色谱-串联质谱（UHPLC-MS/MS）快速同时测定生姜中姜辣素类和姜黄素类营养成分的分析方法，具体包括6-姜酚、8-姜酚、10-姜酚、6-姜烯酚、8-姜烯酚、10-姜烯酚、四氢姜黄素、姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素10种目标物。采用ZORBAX RRHD Eclipse Plus C18色谱柱（100 mm×2.1 mm，1.8 μm）分离，以0.1%（v/v）甲酸水溶液和0.1%（v/v）甲酸甲醇溶液为流动相进行梯度洗脱，采用电喷雾电离（ESI）源、正离子和多反应监测（MRM）模式对目标物进行定性确证和定量分析。10种营养成分的线性相关系数（r）均≥ 0.9995，方法的定量限为0.10~7.71 μg/L，样品基质在3个水平下的平均加标回收率为82.8%~115.3%，相对标准偏差（RSD）为0.58%~11.49%。分析结果显示，生姜中10种营养成分均有检出，其中6-姜酚的含量最高且集中分布于373.35~702.48 mg/kg。该法简便快速，准确可靠，适用于生姜中姜辣素类和姜黄素类营养成分的分析，可为生姜质量鉴定和控制提供技术手段。

关键词: 超高效液相色谱-串联质谱, 姜酚, 姜烯酚, 姜黄素, 营养成分, 生姜

Abstract: An analytical method by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed for the simultaneous determination of the two types of nutrients, viz. gingerol-related compounds and curcuminoids in ginger, including 6-gingerol, 8-gingerol, 10-gingerol, 6-shogaol, 8-shogaol, 10-shogaol, tetrahydrocurcumin, curcumin, demethoxycurcumin and bisdemethoxycurcumin. The 10 target compounds were separated on a ZORBAX RRHD Eclipse Plus C18 column (100 mm×2.1 mm, 1.8 μm) using gradient elution with 0.1% (v/v) formic acid aqueous solution and methanol containing 0.1% (v/v) formic acid as the mobile phases. Qualitative confirmation and quantitative analysis of the target compounds were performed using an electrospray ionization (ESI) source in the positive ion and multiple reaction monitoring (MRM) modes. The linear correlation coefficients (r) of the 10 nutrients were ≥ 0.9995. The limits of quantification were 0.10-7.71 μg/L. The average spiked recoveries of the samples at the three levels were 82.8%-115.3%, the relative standard deviations (RSDs) were 0.58%-11.49%. The results showed that all the 10 nutrients in ginger were detected, and the content of 6-gingerol was the highest at 373.35-702.48 mg/kg. This method is convenient, rapid, accurate and reliable, and is suitable for the analysis of gingerol-related and curcuminoids in ginger. It provides a technical means for the quality identification and control of ginger.

Key words: ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), gingerol, shogaol, curcumin, nutrients, ginger

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