仿生聚合物材料用于糖肽的高选择性富集

doi:10.3724/SP.J.1123.2019.03028

色谱 ›› 2019, Vol. 37 ›› Issue (8): 845-852.DOI: 10.3724/SP.J.1123.2019.03028

仿生聚合物材料用于糖肽的高选择性富集

陈成^1,2, 康虹健^1,2, 张小菲¹, 李秀玲¹, 梁鑫淼¹

1. 中国科学院大连化学物理研究所, 中国科学院分离分析化学重点实验室, 辽宁大连 116023;
2. 中国科学院大学北京 100049

收稿日期:2019-03-22 出版日期:2019-08-08 发布日期:2015-07-30
通讯作者: 李秀玲
基金资助:
国家自然科学基金项目（21775148）.

Biomimetic polymer material for glycopeptide high selective enrichment

CHEN Cheng^1,2, KANG Hongjian^1,2, ZHANG Xiaofei¹, LI Xiuling¹, LIANG Xinmiao¹

1. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2019-03-22 Online:2019-08-08 Published:2015-07-30
Supported by:
National Natural Science Foundation of China (No. 21775148).

摘要/Abstract

摘要： 蛋白质糖基化分析对深入认识其生物功能和发现生物标志物意义重大。由于生物样品中糖肽的丰度低，糖肽的富集至关重要。该文合成了一种以色氨酸为功能基团的新型仿生聚合物材料（简称Poly-Trp），并用于糖肽的高选择性富集。扫描电子显微镜、热分析仪和红外光谱仪等表征证明Poly-Trp已成功合成。通过考察溶液条件变化对糖肽在Poly-Trp保留的影响，发现糖肽在Poly-Trp上的保留分别随着溶液中乙腈浓度的降低和酸度的升高而变弱。且Poly-Trp对牛胎球蛋白糖肽的富集选择性高于商品化ZIC-HILIC和氨基硅胶，能抵抗物质的量倍数为100的牛血清白蛋白的干扰。该研究中仿生聚合物材料的设计为糖肽富集材料提供了新思路和新方法。

关键词: 仿生材料, 聚合物材料, 色氨酸, 糖肽富集

Abstract: The study of protein glycosylation is important to deepening the current understanding of its biological functions as well as to elucidate novel biomarkers of glycosylation. However, glycopeptides must be enriched prior to analysis due to their naturally low abundance. In this study, tryptophan functionalized polymer materials (denoted as Poly-Trp) were synthesized to serve as a novel biomimetic polymers. The resulting materials were characterized by various methods including scanning electron microscopy, thermal analysis, and infrared spectrometry, validating the successful synthesis of Poly-Trp. The retention of glycopeptides on Poly-Trp was investigated revealing that the retention ability decreased as the acetonitrile content of the mobile phase decreased and its acidity increased. Poly-Trp exhibited higher enrichment selectivity for glycopeptides from bovine fetuin than the commercial material ZIC-HILIC, and aminated silica which could defect the interference of 100-fold amount of substance ratios of bovine serum albumin. Poly-Trp is expected to have further applications in the purification of biological samples for the study of glycosylation.

Key words: biomimetic materials, glycopeptide enrichment, polymer materials, tryptophan

中图分类号:

O658

陈成, 康虹健, 张小菲, 李秀玲, 梁鑫淼. 仿生聚合物材料用于糖肽的高选择性富集[J]. 色谱, 2019, 37(8): 845-852.

CHEN Cheng, KANG Hongjian, ZHANG Xiaofei, LI Xiuling, LIANG Xinmiao. Biomimetic polymer material for glycopeptide high selective enrichment[J]. Chinese Journal of Chromatography, 2019, 37(8): 845-852.

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仿生聚合物材料用于糖肽的高选择性富集

Biomimetic polymer material for glycopeptide high selective enrichment

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