色谱

色谱 ›› 2019, Vol. 37 ›› Issue (10): 1112-1117.DOI: 10.3724/SP.J.1123.2019.03031

• 研究论文 • 上一篇    下一篇

高效液相色谱法测定鸡组织中苯并咪唑类药物残留标志物

邹游, 邵琳智, 吴映璇   

  1. 广东检验检疫技术中心, 广东 广州 510623
  • 收稿日期:2019-03-25 出版日期:2019-10-08 发布日期:2020-12-11
  • 通讯作者: 邵琳智,Tel:(020)38290337,E-mail:slz9741430@sina.com.
  • 基金资助:
    农业行业标准制定和修订项目（2017-108）.

Determination of benzimidazoles residue markers in chicken tissue by high performance liquid chromatography

ZOU You, SHAO Linzhi, WU Yingxuan   

  1. Guangdong Inspection and Quarantine Technology Center, Guangzhou 510623, China
  • Received:2019-03-25 Online:2019-10-08 Published:2020-12-11
  • Supported by:
    Project of Agricultural Industry Standards Development and Revision (No. 2017-108).

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摘要： 建立了高效液相色谱同时测定鸡肉、鸡肝脏和鸡肾脏中11种苯并咪唑类药物残留标志物的方法。样品采用乙腈提取，经正己烷脱脂后以MCX固相萃取小柱净化，净化液浓缩处理后采用Atlantis T3色谱柱分离，以甲醇-1%（v/v）乙酸水溶液为流动相进行梯度洗脱，紫外检测波长为292 nm，外标法定量检测。目标化合物在25~1000 μg/L范围内线性良好，定量限为50 μg/kg；在不同添加水平下目标化合物的回收率为70.91%~103.21%，相对标准偏差为1.48%~10.08%（n=6），精密度和准确度均符合要求，能够满足鸡组织中苯并咪唑类药物残留标志物的检测要求。

关键词: 高效液相色谱, 固相萃取, 苯并咪唑类药物残留标志物, 鸡组织

Abstract: A method was developed for the simultaneous determination of 11 benzimildazoles residue markers in chicken meat, liver and kidney samples by high performance liquid chromatography (HPLC). The chicken tissue sample was extracted with acetonitrile, defatted with n-hexane, and purified with an MCX solid-phase extraction cartridge. Chromatographic separation was performed on an Atlantis T3 column with the gradient elution of methyl alcohol-1% (v/v) acetic acid aqueous solution as the mobile phases at a flow rate of 1.0 mL/min. The detection wavelength was set at 292 nm. Analytes were quantified by the external standard method. The linearities of the calibration curves were well in the range of 25-1000 μg/L, and the limit of quantification was 50 μg/kg. The recoveries at different spiked levels in all the three matrices ranged from 70.91% to 103.21%, and the relative standard deviations (RSDs) were between 1.48% and 10.08% (n=6). The developed method is characterized by an excellent purification effect, sensitivity and accuracy, and can be used for the determination of benzimidazoles residue markers in chicken tissue samples.

Key words: high performance liquid chromatography (HPLC), solid phase extraction(SPE), benzimidazoles residue markers, chicken tissue

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