色谱

色谱 ›› 2020, Vol. 38 ›› Issue (7): 868-874.DOI: 10.3724/SP.J.1123.2020.02019

• 技术与应用 • 上一篇    

热辅助解吸-介质阻挡放电离子化质谱快速检测磺胺类药物

叶倩1, 朱秋梦1, 周峰1, 吴剑平2, 严凤2, 赵鹏1,*(), 闻路红1,3   

  1. 1 宁波大学, 宁波高等技术研究院, 浙江 宁波 315211
    2 上海市兽药饲料检测所, 上海 201103
    3 广州市华粤行仪器有限公司, 广东 广州 511400
  • 收稿日期:2020-02-20 出版日期:2020-07-08 发布日期:2020-12-10
  • 通讯作者: 赵鹏
  • 作者简介:赵鹏.Tel:87609856, E-mail:zhaopeng@nbu.edu.cn
  • 基金资助:
    国家重点研发计划(2018YFC1603504);宁波市自然科学基金(2016A610055);番禺区创新创业领军团队(2017-R01-5)

Rapid detection of sulfonamides by heat assisted desorption-dielectric barrier discharge ionization mass spectrometry

YE Qian1, ZHU Qiumeng1, ZHOU Feng1, WU Jianping2, YAN Feng2, ZHAO Peng1,*(), WEN Luhong1,3   

  1. 1 Ningbo University, Ningbo Research Institute of Advanced Technologies, Ningbo 315211, China
    2 Shanghai Institute for Veterinary Drug&Feedstuffs Control, Shanghai 201103, China
    3 Hua Yue Enterprise Holdings Ltd., Guangzhou 511400, China
  • Received:2020-02-20 Online:2020-07-08 Published:2020-12-10
  • Contact: ZHAO Peng
  • Supported by:
    National Key R & D Program of China(2018YFC1603504);Ningbo Natural Science Foundation(2016A610055);the Entrepreneurial and Innovative Leading Team of Panyu District(2017-R01-5)

RichHTML

5

PDF

70

摘要：

磺胺类药物在家禽养殖行业中的滥用是一个极其严重的问题。该文采用一种热辅助解吸介质阻挡放电离子化质谱技术（HAD-DBDI-MS），通过对其加热温度等参数的优化，建立了对磺胺吡啶、磺胺间甲氧嘧啶、磺胺喹噁啉、磺胺胍及磺胺甲噁唑等磺胺类药物的快速检测方法，并通过二级质谱进行结构鉴定；与单独使用DBDI-MS相比，HAD-DBDI-MS的检出限降低了1~2个数量级。对鸡肉模拟加标进行了直接快速检测应用，实现了鸡肉中模拟添加磺胺吡啶的检出；通过在加热时引入甲醇辅助挥发，实现了对饲料模拟加标压片样品中磺胺胍、磺胺甲噁唑的检出。结果表明，HAD-DBDI-MS为禽类肉制品和饲料中磺胺类药物的快速检测提供了一种新的方法。

关键词: 介质阻挡放电离子化, 热辅助解吸, 磺胺, 禽类肉制品, 饲料

Abstract:

Sulfanilamide is a common antibacterial drug that is used in clinical practice and in the industry. However, its abuse has emerged as a serious problem in the aquaculture industry, and more predominantly in the poultry industry, where it is administered for therapeutic purposes or as a growth promoter. In this study, a novel technical platform, heat-assisted desorption-dielectric barrier discharge ionization mass spectrometry (HAD-DBDI-MS), was used to detect sulfanilamide in situ. A method for the rapid identification of five typical sulfanilamide drugs sulfamopyridine, sulfamethoxan, sulfamethoxoline, sulfamidine, and sulfamethoxazole was established after optimizing a series of parameters. Secondary mass spectrometry was used to distinguish the sulfanilamide drugs from one another and from other isomers. Our HAD-DBDI-MS method enhanced the sulfanilamide sensitivities by reducing the limits of detection by about 1-2 orders of magnitude compared to those obtained with the DBDI-MS method. As further research, the profiles of chicken feed with added sulfamopyridine standard as well those of commercial feed with added sulfamidine and sulfamethoxazole were obtained using HAD-DBDI-MS respectively. With the HAD-DBDI-MS method, sulfamopyridine could be directly detected in the complex chicken meat matrix. However, satisfactory results were not obtained when direct HAD-DBDI-MS was used for sulfamidine and sulfamethoxazole detection in the feed matrix. The identification ability was improved when methanol was introduced as the assisting solvent. In this method, chicken feed spiked with sulfamidine and sulfamethoxazole standards were pressed into tablets, and methanol was dropped on the tablet surface. The experimental data indicate that HAD-DBDI-MS is suitable for the rapid identification of sulfanilamide drugs in poultry feed and meat products, thus showing potential value as a detection tool for future application.

Key words: dielectric barrier discharge ionization, heat assisted desorption, sulfanilamides, poultry meat products, feed