色谱

色谱

• “多维色谱”专栏 • 上一篇    下一篇

鼠肝蛋白质组的纳升级多维液相色谱分离

王彦,高明霞,谷雪,张祥民   

  1. Department of Chemistry, Fudan University, Shanghai 200032, China
  • 收稿日期:2004-06-23 修回日期:1900-01-01 出版日期:2005-01-30 发布日期:1989-03-25
  • 通讯作者: 张祥民
  • 基金资助:

Nano Flow Multidimensional Liquid Chromatography for Proteome Analysis of Rat Liver

WANG Yan, GAO Mingxia, GU Xue, ZHANG Xiangmin   

  1. Department of Chemistry, Fudan University, Shanghai 200032, China
  • Received:2004-06-23 Revised:1900-01-01 Online:2005-01-30 Published:1989-03-25

PDF

697

被引次数

16

摘要： 搭建了一个纳升级的二维液相色谱分离平台(nano-2D-LC),该平台可以自动完成进样、除盐、分离及鉴定。以离子交换色谱(SCX)为第一维,反相液相色谱(RPLC)为第二维,对鼠肝组织的蛋白质组进行了研究。SCX采用阶梯式洗脱,RPLC运用线性梯度洗脱,以200 nL/min的速度进行分离,峰容量可达620。

关键词: 蛋白质组, 多维液相色谱, 纳升级 , 鼠肝

Abstract: A nano flow comprehensive multidimensional liquid chromatography was developed. By using this separation platform, complex samples can be injected, desalted, separated and analyzed in complete automatization. Coupling of the strong cation-exchange (SCX) with capillary reversed-phase liquid chromatography (cRPLC) is described for proteomics of rat liver. A step gradient was applied for SCX column, and a linear gradient was applied for RPLC. The flow rate through the nano scale analytical column during the separation process was maintained at 200 nL/min. The peak capacity for the nano-2D-LC was up to 620 under these conditions.

Key words: multidimensional liquid chromatography, nano flow , rat liver, proteomics