色谱

色谱

• “多维色谱”专栏 • 上一篇    下一篇

全二维液相色谱串联质谱用于银杏叶提取物成分分析的研究

陈学国1,孔亮1,盛亮洪2,厉欣1,邹汉法1   

  1. 1. National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China; 2. School of Traditional Chinese Medicines, China Pharmaceutical University, Nanjing 210038, China
  • 收稿日期:2004-06-01 修回日期:1900-01-01 出版日期:2005-01-30 发布日期:1989-03-25
  • 通讯作者: 邹汉法
  • 基金资助:

Applications of Two-Dimensional Liquid Chromatography Coupled to Mass Spectrometry for the Separation and Identification of Compounds in Ginkgo biloba　Extracts

CHEN Xueguo1, KONG Liang1, SHENG Lianghong2, LI Xin1, ZOU Hanfa1   

  1. 1. National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China; 2. School of Traditional Chinese Medicines, China Pharmaceutical University, Nanjing 210038, China
  • Received:2004-06-01 Revised:1900-01-01 Online:2005-01-30 Published:1989-03-25

PDF

776

被引次数

135

摘要： 建立了全二维液相色谱串联质谱分离分析模式,将质脂体色谱柱和ODS反相色谱柱作为二维分析色谱柱,二者通过一个连有两个0.5 mL定量环的八通阀耦联。质脂体色谱柱上的馏分在反相色谱柱上分离后,直接进入紫外-检测器,然后经分流器分流后进入大气压电离质谱。将该体系用于银杏叶提取物的组成研究,共检测到至少41个组分,结合紫外-可见光谱和质谱信息,其中13个组分初步鉴定为银杏内酯B、银杏内酯C、白果内酯、槲皮素芸香糖苷、槲皮素、槲皮素-3-O-β-D-葡萄糖基(1-2)-α-L-鼠李糖苷、槲皮素-3-O-β-D-葡萄糖苷、异鼠李素、山柰酚-3-O-β-D-芸香糖基(1-2)-α-L-鼠李糖苷、异鼠李素-3-O-β-D-芸香糖苷、山柰酚-3-O-β-D-葡萄糖苷、山柰酚和山柰酚-3-O-β-D-芸香糖苷。

关键词: 全二维液相色谱, 银杏叶提取物, 质谱, 中药

Abstract: A comprehensive two-dimensional liquid chromatographic separation system based on the combination of an immobilized liposome chromatographic (ILC) column and an ODS column was developed for the separation of components in Ginkgo biloba, a traditional Chinese medicine. Two columns were coupled by a two-position, eight-port valve equipped with two storage loops, and the system was controlled by a computer. The effluent was detected both by a diode array detector and by an atmospheric pressure chemical ionization (APCI) mass spectrometer. Under the optimization separation conditions with the separation system, more than 41 components in the methanol extract of Ginkgo biloba were resolved. According to their UV and mass spectra, 13 of them were preliminarily identified as ginkgolide B, ginkgolide C, bilobalide, rutin, quercetin, quercetin-3-O-β-D-glucosyl (1-2)-α-L-rhamnoside, quercetin-3-O-β-D-glucoside, isorhamnetin, kaempferol-3-O-β-D-glucosyl (1-2)-α-L-rhamnoside, isohamnetin-3-O-β-D-rutinoside, kaempferol-3-O-β-D-glucoside, kaempferol, kaempferol-3-O-β-D-rutinoside.

Key words: Ginkgo biloba extracts, mass spectrometry, traditional Chinese medicines , comprehensive two-dimensional liquid chromatography