色谱

色谱

• 研究简报 • 上一篇    下一篇

芦荟色酮的高速逆流色谱分离制备方法研究

潘霞,曹学丽,董银卯,赵华   

  1. Beijing Key Laboratory of Plant Resources Research and Development, School of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100037, China
  • 收稿日期:2004-02-16 修回日期:1900-01-01 出版日期:2005-01-30 发布日期:1989-03-25
  • 通讯作者: 曹学丽
  • 基金资助:

Preparative Isolation and Purification of Cinnamoyl-C-Glycoside Chromone from Aloe Vera by High-

PAN Xia, CAO Xueli, DONG Yinmao, ZHAO Hua   

  1. Beijing Key Laboratory of Plant Resources Research and Development, School of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100037, China
  • Received:2004-02-16 Revised:1900-01-01 Online:2005-01-30 Published:1989-03-25

PDF

737

被引次数

33

摘要： 芦荟色酮是在芦荟叶中特有的一类具有抗炎和抑制酪氨酸酶等作用的活性物质。以芦荟全叶为原料,经过一系列的预处理手段,从脱色剂活性炭中获得芦荟色酮粗提物,再经过溶剂分配和富集后采用高速逆流色谱（HSCCC）对其中的色酮成分进行分离纯化。研究结果表明,采用氯仿-甲醇-水(体积比为4∶3∶2)混合溶液和二氯甲烷-甲醇-水(体积比为5∶4∶2)混合溶液作为溶剂分离系统,经过两步HSCCC可以分离纯化出色谱纯度在95％以上的芦荟色酮单体。经过紫外检测、快原子轰击质谱及核磁共振等方法的结构分析鉴定,证实分离所得物质为肉桂酰基-C-葡萄糖甙芦荟色酮。

关键词: 分离纯化 , 高速逆流色谱, 芦荟色酮

Abstract: Aloe chromone is a group of anti-inflammatory and anti-tyrosinase constituents found in aloe vera leaves. High-speed countercurrent chromatography (HSCCC) is reported for the preparative isolation and purification of a chromone from aloe vera. The crude extract was obtained by a series of pretreatment of aloe vera leaves and extracted from decolorizing active carbon with methanol. Then the extract was distributed between dichloromethane and water, and the organic part was then subjected to HSCCC for the isolation of chromone constituents. The chromone compounds with a high performance liquid chromatographic grade (>95%) was isolated through two step HSCCC separations by employing two solvent systems composed of chloroform-methanol-water and dichloromethane-methanol-water at volume ratios of 4/3/2 and 5/4/2, respectively. The chromone was finally identified as cinnamoyl-C-glycoside chromone by ultraviolet (UV), fast atom bombardment mass spectrometry (FAB-MS), nuclear magnetic resonance (1H NMR and 13C NMR).

Key words: aloe chromone, isolation and purification , high-speed countercurrent chromatography