色谱

色谱

• 研究简报 • 上一篇    下一篇

反相高效液相色谱法同时测定4种氟喹诺酮类药物在鸡可食性组织中的残留

董琳琳1,刘艳华1,汪霞1,仲锋1,彭莉2,岳秀英2,高岚2   

  1. 1. China Institute of Veterinary Drug Control, Beijing 100081, China; 2. Sichuan Province Institute of Veterinary Drug Control, Chengdu 610041, China
  • 收稿日期:2004-05-13 修回日期:1900-01-01 出版日期:2005-05-30 发布日期:1988-09-25
  • 通讯作者: 仲锋
  • 基金资助:

Simultaneous Determination of Four Fluoroquinolone Residues in Edible Chicken Tissues by

DONG Linlin1, LIU Yanhua1, WANG Xia1, ZHONG Feng1,　PENG Li2, YUE Xiuying2,　GAO Lan2   

  1. 1. China Institute of Veterinary Drug Control, Beijing 100081, China; 2. Sichuan Province Institute of Veterinary Drug Control, Chengdu 610041, China
  • Received:2004-05-13 Revised:1900-01-01 Online:2005-05-30 Published:1988-09-25

PDF

635

被引次数

178

摘要： 建立了一种可同时测定鸡可食性组织中环丙沙星、达氟沙星、恩诺沙星及沙拉沙星等多种残留的反相高效液相色谱 -荧光分析法。鸡的肌肉、皮和脂、肝、肾等4种组织经不同pH值的磷酸二氢钾缓冲溶液匀浆提取,上清液通过C18固相萃 取柱净化,以流动相洗脱。洗脱液经液相色谱分离后,用荧光检测器进行检测(激发波长280 nm, 发射波长450 nm),外标 法定量。对鸡的4种组织进行添加回收率测定,结果显示方法在添加水平为20~300 μg/kg时药物的回收率约为53.9%～93.4%,批间回收率测定值的相对标准偏差低于23%;环丙沙星、恩诺沙星、沙拉沙星 的定量检出限为20 μg/kg,达氟沙星为4μg/kg。方法简单、快速，能满足常规兽药残留检测的需要。

关键词: 残留, 达氟沙星, 恩诺沙星, 高效液相色谱法, 环丙沙星, 鸡可食性组织 , 沙拉沙星

Abstract: A reversed-phase high performance liquid chromatographic method with fluorescence detection was developed for the simultaneous analysis of ciprofloxacin, danofloxacin, enrofloxacin and sarafloxacin residues in edible chicken tissues. The analytes were extracted from chicken muscle, skin and fat, liver, kidney by aqueous potassium dihydrogenphosphate of different pH values through homogenization. The supernatant of the extract was applied onto a C18 solid phase extraction cartridge for clean-up. The separation was achieved on a C18 column, and the detection was performed with a fluorescence detector (excitation at 280 nm and emission at 450 nm). The four fluoroquinolones were analyzed in spiked samples of four chicken tissues with mean recoveries in the range of 53.9%-93.4% at spiked levels of 20-300 μg/kg. The relative standard deviations of inter-assay were no more than 23%. The detection limits of quantification were 20μg/kg for ciprofloxacin, enrofloxacin and sarafloxacin and 4μg/kg for danofloxacin. The method is simple, fast, and sufficient for routine analysis.

Key words: , ciprofloxacin, danofloxacin, enrofloxacin, high performance liquid chromatography