色谱

色谱

• 研究简报 • 上一篇    下一篇

丹参药材水溶性成分的高效液相色谱指纹图谱研究

周欣1,王道平1,梁光义1,张雪琴1,张中林2   

  1. 1. The Key Laboratory of Chemistry for Natural Products of Guizhou Province and The Chinese Academy of Sciences, Guiyang 550002, China; 2. Guizhou Shenqi Pharmaceutical Co. Ltd., Guiyang 550002, China
  • 收稿日期:2004-05-30 修回日期:1900-01-01 出版日期:2005-05-30 发布日期:1988-09-25
  • 通讯作者: 周欣
  • 基金资助:

Study on Fingerprint Chromatograms of Water-Soluble Constituents of Salvia miltiorrhiza Bge. by

ZHOU Xin1, WANG Daoping1, LIANG Guangyi1, ZHANG Xueqin1 , ZHANG Zhonglin2   

  1. 1. The Key Laboratory of Chemistry for Natural Products of Guizhou Province and The Chinese Academy of Sciences, Guiyang 550002, China; 2. Guizhou Shenqi Pharmaceutical Co. Ltd., Guiyang 550002, China
  • Received:2004-05-30 Revised:1900-01-01 Online:2005-05-30 Published:1988-09-25

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摘要： 采用高效液相色谱法(HPLC)对贵州铜仁丹参基地的丹参药材进行了指纹图谱研究。实验采用Zorbax SB-C18色谱柱 (5 μm, 4.6 mm i.d.×250 mm),以乙腈-0.4%(体积分数)冰醋酸水溶液为流动相,乙腈的体积分数在70 min内由0线性 增加到40%;流速1.0 mL/min;柱温25 ℃;检测波长254 nm。以19个主要共有峰为评价指标,采用国家药品监督管理局推荐 的“计算机辅助中药指纹图谱相似度计算软件”计算处理,建立了铜仁地区丹参药材的HPLC共有指纹图谱。该法操作简单 ,精密度、稳定性和重现性良好,有助于加强丹参药材的质量控制。

关键词: 丹参 , 高效液相色谱, 水溶性成分, 指纹图谱

Abstract: The fingerprint chromatograms were established for the quality evaluation of Salvia miltiorrhiza Bge. by high performance liquid chromatography. The analysis was performed on a Zorbax SB-C18 column (5 μm, 4.6 mm i.d.×250 mm) with acetonitrile-water (containing 0.4% (v/v) acetic acid ) (the volume fraction of acetonitrile from 0 to 40% within 70 min) as mobile phase at a flow rate of 1.0 mL/min, and at a column temperature of 25 ℃.The detection wavelength was 254 nm. The relative standard deviations of relative retention values and relative peak areas were less than 3%. The mutual fingerprint of the extract of Salvia miltiorrhiza Bge. collected from Danshen Base in Tongren City of Guizhou Province was established by using the similarity calculation software of Chinese herbal fingerprint. The method is reliable and can be helpful in effectively controlling the quality of Salvia miltiorrhiza Bge.

Key words: fingerprint, Salvia , water-soluble constituents, high performance liquid chromatography