色谱

色谱

• 研究简报 • 上一篇    下一篇

汽车漆皮的气相色谱指纹图谱比对方法及应用研究

李辰,梁冰,师彦平,蒋生祥,欧庆瑜

  

  1. Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences, Lanzhou 730000, China
  • 收稿日期:2004-12-02 修回日期:1900-01-01 出版日期:2005-11-30 发布日期:1987-12-25
  • 通讯作者: 李辰

Investigation on Comparison Method of Chromatographic Fingerprints of Lacquer Coat of Cars and Its Application

LI Chen, LIANG Bing, SHI Yanping, JIANG Shengxiang, OU Qingyu   

  1. Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences, Lanzhou 730000, China
  • Received:2004-12-02 Revised:1900-01-01 Online:2005-11-30 Published:1987-12-25

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摘要：

采用在线热解吸/毛细管气相色谱联用方法建立了对汽车漆皮指纹图谱进行比对的方法,并将该方法应用于实际样品的鉴定。该方法通过将一定大小和质量的漆皮样品置于热解吸炉中进行加热解吸,自漆皮中解吸出来的挥发性有机物在色谱仪上得到分离检测。通过直观比较两张漆皮图谱的峰形轮廓是否一致进行初步判断,对外形相似的图谱进一步计算二者谱峰的重叠率,并以相对保留时间比值rt2/rt1和相对峰面积比值rA2/rA1来考察待比较图谱的相似度。当谱峰重叠率大于90%,rt2/rt1和rA2/rA1的相似度分别大于99%和70%时,可认定待比较的两个漆皮样品来自同一辆车。该方法操作简便,谱峰识别能力强,实现了对指纹图谱的定量表征描述,使漆皮的仪器分析数据成为法律上可采信的有力物证之一。

关键词: 比对, 漆皮 , 在线热解吸/气相色谱联用法, 指纹图谱

Abstract:

The comparison method of fingerprints of the lacquer coat of cars (LCC) was established by using thermal desorption instrument with gas chromatography. The actual LCC samples were also analyzed. The samples were cut out to proper size and placed in the desorption furnace of the thermal desorption instrument. The volatile organic compounds in LCC were desorbed from the lacquer coat samples in the furnace under the chosen temperature, then separated in the capillary column and detected on a flame ionization detector of gas chromatography. The incipient judgment whether the two fingerprints of LCC were the same can be made from the contour and figure of the chromatograms. To make farther study of the two similar fingerprints, the overlap ratio of the peaks and relative retention values were given in the article. The two LCC samples can be regarded as the same if the overlap ratio of peaks was more than 90%, and the similarity of the ratio of relative retention times rt2 and rt1 and relative peak areas rA2 and rA1 in the two fingerprints were more than 99% and 70%, respectively. The method is good in repeatability and is easily carried out. The peaks in the fingerprint can be readily recognized. The fingerprint was characterized quantitatively. The method can be used in the department of traffic police and the comparison result can be used as material evidence in the court.

Key words: comparison, fingerprints, lacquer coat , online using of thermal desorption with gas chromatography