色谱

色谱

• 研究论文 • 上一篇    下一篇

反相高效液相色谱法同时测定祛痘类化妆品中的禁用物质

武婷1,王超2,李楠1   

  1. 1.College of Science, China Agricultural University, Beijing 100094, China; 2.Chinese Academy of Inspection and Quarantine, Beijing 100025, China
  • 收稿日期:2006-01-12 修回日期:2006-04-10 出版日期:2006-11-30 发布日期:1986-06-25
  • 通讯作者: 李楠

Simultaneous Determination of Inhibitive Components in Anti-Acne Cosmetics by Reversed-Phase High Performance Liquid Chromatography

WU Ting1, WANG Chao2, LI Nan1   

  1. 1.College of Science, China Agricultural University, Beijing 100094, China; 2.Chinese Academy of Inspection and Quarantine, Beijing 100025, China
  • Received:2006-01-12 Revised:2006-04-10 Online:2006-11-30 Published:1986-06-25
  • Contact: LI Nan

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982

被引次数

15

摘要： 建立了用反相高效液相色谱法(RP-HPLC)同时测定祛痘类化妆品中禁用物质安体舒通、过氧化苯甲酰和维A酸的含量。采用甲醇超声提取,HPLC法分离测定。3种被测物在11 min内均得到良好的分离。在1～200 mg/L范围内其浓度与峰面积呈良好的线性关系(r≥0.9999);在添加质量浓度为1～10 mg/L时,回收率为88.2%～106.7%,相对标准偏差小于3.1%;最低检出限(S/N=3)为安体舒通0.101 μg,过氧化苯甲酰0.100 μg,维A酸0.107 μg。该法简便、快速、准确,可用于祛痘类化妆品中以上3种禁用物质的检测。

关键词: 安体舒通, 反相高效液相色谱法, 过氧化苯甲酰, 化妆品 , 祛痘, 维A酸

Abstract: The inhibitive components in anti-acne cosmetics including spironolactone, benzoyl peroxide, and tretinoin were simultaneously determined by reversed-phase high performance liquid chromatography (RP-HPLC). The cosmetics were extracted with methanol by microwave and analyzed by high performance liquid chromatography. The HPLC conditions were as follows: Kromasil C18 column (4.6 mm i.d.×250 mm, 5 μm), methanol and phosphate buffer as mobile phase with gradient elution at a flow rate of 1.0 mL/min, UV detection at 265 nm. Three components were separated completely within 11 min. The calibration curves of the three compounds were linear (r>0.9999) between 1 and 200 mg/L. The average recoveries were from 88.2% to 106.7% with relative standard deviations lower than 3.1%. The detection limits (S/N=3) were 0.101 μg for spironolactone, 0.100 μg for benzoyl peroxide, and 0.107 μg for tretinoin. The method is simple and rapid with high accuracy, and suitable for the determination of the 3 inhibitive components in anti-acne cosmetics.

Key words: anti-acne, benzoyl peroxide, cosmetics , spironolactone, tretinoin, reversed-phase high performance liquid chromatography （RP-HPLC）