色谱

色谱

• 研究论文 • 上一篇    下一篇

色谱指纹图谱在苹果酒质量评价中的应用

徐抗震,宋纪蓉,任莹辉,马海霞,黄洁,杜晓丹   

  1. Shaanxi Key Laboratory of Physical-Inorganic Chemistry, Department of Food Science
    and Engineering, Northwest University, Xi’an 710069, China
  • 收稿日期:2006-04-13 修回日期:2006-11-02 出版日期:2007-01-30 发布日期:1986-03-25
  • 通讯作者: 宋纪蓉

Application of Fingerprint Chromatogram in Quality Assessment of Apple Cider

XU Kangzhen, SONG Jirong, REN Yinghui, MA Haixia, HUANG Jie, DU Xiaodan   

  1. Shaanxi Key Laboratory of Physical-Inorganic Chemistry, Department of Food Science
    and Engineering, Northwest University, Xi’an 710069, China
  • Received:2006-04-13 Revised:2006-11-02 Online:2007-01-30 Published:1986-03-25
  • Contact: SONG Jirong

PDF

732

摘要： 采用反相高效液相色谱-电化学检测法研究了14种苹果酒样品的指纹图谱。以标准品绿原酸进行定位,通过对图谱分析和相对保留时间计算，确定了8个共有峰。根据共有峰的峰面积用相关系数法和向量夹角余弦法计算相似度，两种方法的计算结果一致。实验结果表明同一厂家生产的苹果酒相似度较好。该法为苹果酒的产品分析提供了有效的微观信息，为苹果酒的质量控制、新产品的研发以及苹果酒行业标准的制定提供一种可行思路。

关键词: 电化学检测, 高效液相色谱, 苹果酒 , 指纹图谱

Abstract: Fingerprints of 14 apple cider samples from different manufacturers were studied using high performance liquid chromatography (HPLC) with an electrochemical detector (ECD). The analysis was carried out on a Zorbax SB-C18 column at 30 ℃ with 2% (v/v) methanol aqueous solution-4%(v/v) acetic acid aqueous solution as mobile phase at a flow rate of 0.8 mL/min. The electrochemical detector was set at 0.7 V. By calculating the relative retention times of certain peaks with chlorogenic acid as the reference standard, 8 common peaks in the samples were analyzed. Relative retention times for the common peaks of various samples were calculated, and the similarities of all the samples were figured out through each peak area with the vectorial angle cosine method and correlative coefficient method. The results indicated that apple cider products of the same manufacturer have good similarity, with the similarities greater than 92.7%. According to this experiment, effectual microcosmic information for apple cider analysis was gained through HPLC and ECD. Moreover, this test method will help the analysis and the control of product quality, the development of new products and the establishment of trade standard.

Key words: apple cider , electrochemical detection, fingerprint, high performance liquid chromatography (HPLC)