色谱

色谱

• 研究论文 • 上一篇    下一篇

制备型高效液相色谱法制备纯化3种辣椒素单体

董新荣1,2,刘仲华2,李本祥1,王坤波2

  

  1. 1.College of Science, Hunan Agricultural University, Changsha 410128, China;
    2.Natural Product Research Center, Hunan Agricultural University, Changsha 410128, China
  • 收稿日期:2007-08-24 修回日期:2007-10-19 出版日期:2008-05-30 发布日期:1984-03-25
  • 通讯作者: 刘仲华

Preparation of three capsaicinoid components using preparative high performance liquid chromatography

DONG Xinrong1,2, LIU Zhonghua2, LI Benxiang1, WANG Kunbo2   

  1. 1.College of Science, Hunan Agricultural University, Changsha 410128, China;
    2.Natural Product Research Center, Hunan Agricultural University, Changsha 410128, China
  • Received:2007-08-24 Revised:2007-10-19 Online:2008-05-30 Published:1984-03-25
  • Contact: LIU Zhonghua

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摘要：

利用制备型反相高效液相色谱法从辣椒素类物质中制备了3种辣椒素单体。在PRC-ODS色谱柱(250 mm×21.5 mm,13 μm)上,以甲
醇-水(体积比为70∶30)为流动相,流速为15 mL/min,采用等度洗脱方式,从80%的辣椒素类物质中制备了3种辣椒素单体。经核磁共振
氢谱(1H NMR)及电子轰击离子源质谱(EI-MS)分析,确认它们分别为降二氢辣椒素、辣椒素和二氢辣椒素,收率分别为60.1%,58.9%和
72.3%。高效液相色谱分析表明所制备的3种化合物的纯度分别达到了98.12%,99.93%及100.7%。

关键词: 二氢辣椒素, 降二氢辣椒素, 辣椒素, 辣椒素类物质 , 制备高效液相色谱法

Abstract:

A method was established for the isolation of three capsaicinoid components from capsaicinoids using
preparative high performance liquid chromatography (Pre-HPLC). A preparative PRC-ODS column (250 mm×21.5 mm, 13
μm) was used in the preparation process. By isocratic elution with methanol-water (70∶30, v/v) as the mobile phase
at a flow rate of 15 mL/min followed by concentration, three capsaicinoid components were obtained from 80%
capsaicinoids, and subsequently identified by 1H NMR （nuclear magnetic resonance） and EI-MS （electron impact-mass
spectrometry） as nordihydrocapsaicin, capsaicin and dihydrocapsaicin, and the purities of the three components
analyzed by HPLC were 98.12%, 99.93% and 100.7% with yields of 60.1%, 58.9% and 72.3%, respectively.

Key words:
dihydrocapsaicin, capsaicin, capsaicinoids , nordihydrocapsaicin, preparative high performance liquid chromatography （Pre-HPLC）