色谱

色谱

• 技术与应用 • 上一篇    下一篇

固相萃取-反相高效液相色谱法同时测定饲料中的角黄素和虾青素

张华,杨鑫,马莺,董爱军,张英春

  

  1. School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China
  • 收稿日期:2007-09-10 修回日期:2007-12-29 出版日期:2008-05-30 发布日期:1984-03-25
  • 通讯作者: 杨鑫

Simultaneous determination of canthaxanthin and astaxanthin in feedstuffs using solid phase extraction-reversed-
phase high performance liquid chromatography

ZHANG Hua, YANG Xin, MA Ying, DONG Aijun, ZHANG Yingchun   

  1. School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China
  • Received:2007-09-10 Revised:2007-12-29 Online:2008-05-30 Published:1984-03-25

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摘要：

建立了一种同时测定饲料中角黄素和虾青素的固相萃取-高效液相色谱法（HPLC）。样品由乙腈提取,经LC-NH2固相萃取小柱净
化,洗脱剂为乙腈-甲苯（体积比为3∶1）,洗脱液被浓缩后进行HPLC分析,色谱柱为ZORBAX Eclipse XDB-C18柱(150 mm×4.6 mm,5 μ
m),流动相为乙腈-甲醇（体积比为95∶5）,流速1.0 mL/min,采用二极管阵列检测器检测,检测波长为474 nm;外标法定量。角黄素和
虾青素的线性范围分别为1.0～30.0 mg/L和1.0～20.0 mg/L,相关系数分别为0.9990和0.9991,回收率为90%～101%,相对标准偏差为
0.62%～3.68%,检出限分别为0.84和0.60 mg/L。该方法简便、快速、准确,可用于饲料中角黄素和虾青素的同时测定。

关键词: 反相高效液相色谱法, 固相萃取, 角黄素, 饲料 , 虾青素

Abstract:

A method was developed for the simultaneous determination of canthaxanthin and astaxanthin in feedstuffs
using reversed-phase high performance liquid chromatography （RP-HPLC）. The sample was extracted by acetonitrile,
and cleaned up by an LC-NH2 column. An Agilent ZORBAX Eclipse XDB-C18 analytical column (150 mm×4.6 mm, 5 μm) was
used and kept at 25 ℃. Acetonitrile-methanol (95∶5, v/v) was used as the mobile phase at a flow rate of 1.0
mL/min. The detection was performed by a diode array detector at 474 nm. The quantitive analysis of external
standard calibration curves was used. The linear ranges of the method for canthaxanthin and astaxanthin were 1.0-
30.0 mg/L (r=0.9990) and 1.0-20.0 mg/L (r=0.9991), respectively. The average recoveries were 90%-101% with the
relative standard deviations of 0.62%-3.68%. The detection limits were 0.84 and 0.60 mg/L for canthaxanthin and
astaxanthin, respectively. The method is simple, precise, sensitive and reproductive. It can be used to determine
the contents of canthaxanthin and astaxanthin in feedstuffs.

Key words:
canthaxanthin, astaxanthin, feedstuffs , reversed-phase high performance liquid chromatography (RP-HPLC), solid phase extraction (SPE)