色谱

色谱

• 研究论文 • 上一篇    下一篇

毛细管离子电泳法定量监测丙酮丁醇梭菌发酵过程中产生的有机酸的浓度变化

程佳懿,汪彤丹,康经武   

  1. Shanghai Institute of Organic Chemistry, the Chinese Academy of Sciences, Shanghai 200032, China
  • 收稿日期:2008-03-25 修回日期:2008-05-05 出版日期:2008-11-30 发布日期:1983-06-25
  • 通讯作者: 康经武
  • 基金资助:
    中国科学院方向性项目基金(06200916G0).

Quantitative monitoring the concentration changes of organic acids in fermentation process of Clostridium acetobutylicum using capillary ion electrophoresis

CHENG Jiayi, WANG Tongdan, KANG Jingwu   

  1. Shanghai Institute of Organic Chemistry, the Chinese Academy of Sciences, Shanghai 200032, China
  • Received:2008-03-25 Revised:2008-05-05 Online:2008-11-30 Published:1983-06-25
  • Contact: KANG Jingwu

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772

被引次数

3

摘要： 建立了一种用于监测丙酮丁醇梭菌发酵过程中产生的3种有机酸(乳酸、乙酸、正丁酸)浓度变化的毛细管离子电泳方法。该方法以对甲氧基苯甲酸为电泳缓冲液,以十六烷基三甲基氯化铵(CTAC)为电渗流改性剂,采用间接紫外检测方式测定发酵过程中产生的有机酸。发酵液经过简单的离心处理后,上清液稀释后直接进样分析。考察了电泳操作条件对分离的影响,对实验条件进行了优化。优化后的分离条件为10 mmol/L对甲氧基苯甲酸（pH 5.8）、0.15 mmol/L CTAC。该方法测定乳酸、乙酸和正丁酸的定量限分别为1.22，0.38和0.58 mg/L；完成一次分析只需要5 min,并具有良好的重现性。该方法已成功地用于丙酮丁醇梭菌的代谢流量分析。

关键词: 丙酮丁醇梭菌, 发酵液 , 毛细管离子电泳法, 有机酸

Abstract: A method for monitoring the concentration changes of organic acids in the fermentation process of Clostridium acetobutylicum by capillary ion electrophoresis has been developed. In this study, 4-methoxybenzoic acid was used as the background electrolyte for the indirect ultraviolet detection, and cetyltrimethylammonium chloride (CTAC) was employed as the electroosmotic flow modifier. The sample of fermentation was simply treated by centrifugation and dilution. The optimal conditions for the separation were established as 10 mmol/L of 4-methoxybenzoic acid solution （pH 5.8） and 0.15 mmol/L of CTAC solution. The limits of quantification for lactate, acetate and n-butyrate were 1.22, 0.38 and 0.58 mg/L, respectively. The method can be successfully used for the metabolic flux analysis of Clostridium acetobutylicum.

Key words: Clostridium acetobutylicum, fermentation liquid , organic acids, capillary ion electrophoresis