色谱

色谱

• 研究论文 • 上一篇    下一篇

西他沙星差向异构体的毛细管电泳分离

袁佩1,林蕾2,范琦1,曾令高3   

  1. 1.Pharmaceutical Department, Chongqing University of Medical Sciences, Chongqing 400016, China;2.College of Sciences, Chongqing Technology and Business University, Chongqing 400020, China;3.Chongqing Institute for Drug Control, Chongqing 400015, China
  • 收稿日期:2005-11-01 修回日期:2006-01-06 出版日期:2006-09-30 发布日期:1986-09-25
  • 通讯作者: 范琦

Separation of Sitafloxacin Epimers by Capillary Electrophoresis

YUAN Pei1, LIN Lei2, FAN Qi1, ZENG Linggao3   

  1. 1.Pharmaceutical Department, Chongqing University of Medical Sciences, Chongqing 400016, China;2.College of Sciences, Chongqing Technology and Business University, Chongqing 400020, China;3.Chongqing Institute for Drug Control, Chongqing 400015, China
  • Received:2005-11-01 Revised:2006-01-06 Online:2006-09-30 Published:1986-09-25
  • Contact: FAN Qi

PDF

779

被引次数

32

摘要： 以γ-环糊精和D-苯丙氨酸为手性选择剂,采用毛细管区带电泳成功地分离了新型抗菌素西他沙星差向异构体。考察了添加剂的种类、浓度和缓冲溶液的pH对毛细管电泳分离西他沙星的影响。分离电压为15 kV,选用60 cm(有效长度52.5 cm)×50 μm i.d.的石英毛细管,缓冲溶液组成为10 mmol/L KH2PO4-K2HPO4(pH 4.5),10 mmol/L CuSO4,20 mmol/L γ-环糊精和 10 mmol/L D-苯丙氨酸。实验结果表明,添加剂的种类和浓度是影响西他沙星手性分离的重要因素,只有当 D-苯丙氨酸、铜离子和γ-环糊精同时存在并达到一定浓度时,西他沙星差向异构体在毛细管电泳中才具有良好的分离效果。该方法可用于西他沙星差向异构体的定量分析。

关键词: 差向异构体 , 毛细管电泳, 西他沙星

Abstract: Sitafloxacin epimers were separated by capillary zone electrophoresis using γ-cyclodextrin(γ-CD) and D-phenylalanine (D-Phe) as chiral selector．The effects of the concentrations of γ-CD, D-Phe, Cu2+ and pH of buffer were investigated．An uncoated fused-silica capillary of 50 μm i.d. and 60 cm (effective length 52.5 cm) was used．The capillary temperature was maintained at 25 ℃．Samples were injected under a pressure of 7 kPa for 5 s and separated at 15 kV．A baseline separation of sitafloxacin epimers was achieved with a background electrolyte of 10 mmol/L KH2PO4-K2HPO4 (pH 4.5), 10 mmol/L CuSO4, 20 mmol/L γ-CD and 10 mmol/L D-Phe．The linear range for sitafloxacin was 32-400 mg/L (0.996)．The relative standard deviations (RSDs) of migration time and peak area were less than 1.9% and 3.8% respectively．This method can be applied in qualitative and quantitative analysis for sitafloxacin epimers.

Key words: epimers , sitafloxacin, capillary electrophoresis (CE)