色谱

色谱

• 研究论文 • 上一篇    下一篇

反相高效液相色谱法同时测定苍术中的两种聚乙炔类化合物

陈炎明1, 2,侴桂新1, 2,王峥涛1, 2

  

  1. 1.Key Laboratory of Standardization of Chinese Medicines, Ministry of Education, Institutes of Chinese
    Material Medica, Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China;
    2.Shanghai R.＆D. Centre for Standardization of Chinese Medicines, Shanghai 201203, China
  • 收稿日期:2006-03-13 修回日期:2006-06-15 出版日期:2007-01-30 发布日期:1986-03-25
  • 通讯作者: 陈炎明

Simultaneous Determination of Polyacetylene Components
in Cangzhu by Reversed-Phase High Performance
Liquid Chromatography

CHEN Yanming1,2, CHOU Guixin1,2, WANG Zhengtao1,2   

  1. 1.Key Laboratory of Standardization of Chinese Medicines, Ministry of Education, Institutes of Chinese
    Material Medica, Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China;
    2.Shanghai R.＆D. Centre for Standardization of Chinese Medicines, Shanghai 201203, China
  • Received:2006-03-13 Revised:2006-06-15 Online:2007-01-30 Published:1986-03-25

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摘要：

采用反相高效液相色谱法同时测定了苍术中的2种主要聚乙炔类成分(苍术素、苍术素醇)。采用Polaris-C18柱,以乙腈-水溶液为流动相进行梯度洗脱,流速1.0 mL/min,紫外检测波长340 nm。2种聚乙炔类化合物的检测限分别为0.069和0.016 μg，回收率为97.4%~104.6%。该方法操作简单,结果准确,重现性好,可用于苍术药材及饮片中2种主要聚乙炔类化合物的同时测定,具有很高的实用价值。

关键词: 苍术, 苍术素, 苍术素醇, 反相高效液相色谱法, 聚乙炔, 中草药

Abstract:

In traditional Chinese medicines, atractylodes rhizome (“Cangzhu”in Chinese)is used for the treatment of rheumatic diseases, digestive disorders, mild diarrhea, night-blindness. According to the Pharmacopoeia of China (2005 Edition), Rhizoma Atractylodis is the dried root of Atractylodes lancea (Thunb.) DC. or Atractylodes chinensis (DC.) Koidz. Polyacetylenes is one group of the most important active components in Cangzhu. A reversed-phase high performance liquid chromatographic method was developed for the simultaneous determination of two main polyacetylene components including atractylodin and atractylodinol in Cangzhu.The chromatographic analysis was carried out using a Polaris-C18 column and the mobile phase of acetonitrile-water with the flow rate of 1.0 mL/min. The detection wavelength was set at 340 nm, and column temperature was set at 25 ℃. The detection limits (S/N=3) were 0.069 and 0.016 μg for atractylodin and atractylodinol, respectively. The recoveries of the two polyacetylene components were found in the range of 97.4%104.6%. Thirteen samples collected were determined which includes three species of atractylodes rhizome, A.lancea, A.chinensis, and A.japonica. This rapid and accurate method has been successfully applied to the simultaneous determination of the two polyacetylene components in Cangzhu.

Key words: atractylodin, atractylodinol, polyacetylene , reversed-phase high performance liquid chromatography (RP-HPLC)