色谱

色谱

• 研究论文 • 上一篇    下一篇

中药复方小续命汤抗阿尔茨海默病有效成分组中6种有效成分的同时测定

李忠红1,2,倪坤仪3,杜冠华1   

  1. 1.Institute of Materia Medica, Chinese Academy of Medical Sciences &Peking Union Medical College,Beijing 100050, China; 2.Jiangsu Institute for Drug Control, Nanjing 210008, China; 3.China Pharmaceutical University, Nanjing 210009, China
  • 收稿日期:2006-03-10 修回日期:2006-06-15 出版日期:2007-01-30 发布日期:1986-03-25
  • 通讯作者: 杜冠华

Simultaneous Analysis of Six Effective Components in the Anti-Alzheimer’s Disease Effective Component Group of Xiao-Xu-Ming Decoction

LI Zhonghong1,2, NI Kunyi3, DU Guanhua1   

  1. 1.Institute of Materia Medica, Chinese Academy of Medical Sciences &Peking Union Medical College,Beijing 100050, China; 2.Jiangsu Institute for Drug Control, Nanjing 210008, China; 3.China Pharmaceutical University, Nanjing 210009, China
  • Received:2006-03-10 Revised:2006-06-15 Online:2007-01-30 Published:1986-03-25
  • Contact: DU Guanhua

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摘要： 建立了用高效液相色谱分析中药复方小续命汤抗阿尔茨海默病有效成分组中各成分的方法,并将其应用于此有效成分组的质量控制。采用ODS色谱柱,以水-甲酸-乙二胺(流动相A，体积比为100∶0.1∶0.1)和甲醇-甲酸(流动相B，体积比为100∶0.05)为流动相,采用梯度洗脱，流速1 mL/min，检测波长240 nm。在上述色谱条件下,可分离测定有效成分组中的6种成分。6种成分均具有良好的线性；其回收率分别为:芍药苷99.1%,升麻苷99.6%,黄芩苷98.4%,5-O-甲基维斯阿米醇苷99.9%,防己诺林碱99.6%,粉防己碱102.0%;相对标准偏差（RSD）分别为1.3%,1.4%,0.4%,0.8%,0.2%,1.4%。所建立的方法简便、快捷,可用于小续命汤有效成分组的质量控制。

关键词: 高效液相色谱法, 抗阿尔茨海默病 , 小续命汤, 有效成分组, 中药复方

Abstract: A method based on high performance liquid chromatography (HPLC) was developed for the quantitative determination of six components in an anti-Alzheimer medicine, Xiao-Xu-Ming Decoction, which is an effective prescription in treating stroke and the sequela of stroke by herbalist doctors for thousands years. The effective component group (ECG) was made according to the results of high-throughput screening, and the curative effect of ECG was validated on aging rats. In this method an ODS column was used. The mobile phase consisted of water-formic acid-ethylenediamine (A; 100∶0.1∶0.1, v/v) and methanol-formic acid (B; 100∶0.05, v/v), eluted with gradient (05 min, 20%B; 5100 min, 20%B40%B; 100140 min, 40%B70%B). The flow rate was 1 mL/min. The detection wavelength was set at 240 nm. Under the above separation conditions, six components belonged to two different categories, indicans and alkaloids, were determined simultaneously. The relationships between the concentrations and the peak areas of these six components were all linear. The recoveries of the six components were 99.1% for paeoniflorin, 99.6% for prim-O-glucosylcimifugin, 98.4% for baicalin, 99.9% for 4′-O-β-D-glucosyl-5-O-methylvisamminol, 99.6% for fangchinoline, and 102.0% for tetrandrine. The relative standard deviations (RSD) were 1.3%, 1.4%, 0.4%, 0.8%, 0.2%, and 1.4%, respectively. This method is simple and reproducible and it can be used for the quality control of the effective component group of Xiao-Xu-Ming Decoction.

Key words: anti-Alzheimer’s disease , effective component group (ECG), traditional Chinese medicine compound prescriptions, Xiao-Xu-Ming Decoction, high performance liquid chromatography (HPLC)