色谱

色谱

• 研究论文 • 上一篇    下一篇

高效液相色谱法同时测定长春花中的文多灵、长春质碱和脱水长春碱

杨蕾,唐中华,祖元刚

  

  1. Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast
    Forestry University, Harbin 150040, China
  • 收稿日期:2007-01-21 修回日期:2007-04-05 出版日期:2007-07-30 发布日期:1985-06-25
  • 通讯作者: 杨蕾

Simultaneous Determination of Vindoline, Catharanthine and
Anhydrovinblastine in Catharanthus roseus by High
Performance Liquid Chromatography

YANG Lei, TANG Zhonghua, ZU Yuangang   

  1. Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast
    Forestry University, Harbin 150040, China
  • Received:2007-01-21 Revised:2007-04-05 Online:2007-07-30 Published:1985-06-25

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摘要：

运用高效液相色谱（HPLC）梯度洗脱同时测定了长春花中的文多灵、长春质碱和脱水长春碱等3种生物碱。色谱柱为Waters 5C18-MS-Ⅱ(4.6 mm×250 mm),流动相为甲醇-1%二乙胺(磷酸调pH至7.3)系统,梯度洗脱,检测波长220 nm,柱温25 ℃。实验结果表明,文多灵、长春质碱和脱水长春碱等3种生物碱分别在0.03～1 mg/mL,0.03～1 mg/mL和0.01～0.5 mg/mL时线性关系良好,平均加样回收率分别为96.8%,97.0%和96.4%,相对标准偏差(RSD)分别为1.53%,1.37%和1.96%。用该法同时检测了不同干旱条件处理下长春花样品中的文多灵、长春质碱和脱水长春碱,方法准确、快速、简便。

关键词: 长春花 , 长春质碱, 高效液相色谱法, 脱水长春碱, 文多灵

Abstract:

A high performance liquid chromatographic (HPLC) method with gradient elution was developed for the simultaneous determination of vindoline, catharanthine and anhydrovinblastine in Catharanthus roseus. The analytes were separated on a Waters 5C18-MS-Ⅱcolumn (4.6 mm×250 mm) and detected at 220 nm. The mobile phases were methanol-1%(v/v) diethylamine solution (adjusted to pH 7.3 with phosphate) for gradient elution at temperature of 25 ℃. The calibration curves for vindoline, catharanthine and anhydrovinblastine showed good linearity in the ranges of 0.03-1 mg/mL (r=0.9997), 0.03-1 mg/mL (r=0.9999) and 0.01-0.5 mg/mL (r=0.9986), respectively. The recoveries were 96.8%, 97.0%, and 96.4% and the relative standard deviations (RSDs) were 1.53%, 1.37%, and 1.96%. The method is accurate, rapid and simple for the determination of vindoline, catharanthine and anhydrovinblastine in Catharanthus roseus.

Key words: anhydrovinblastine, catharanthine, Catharanthus roseus , vindoline, high performance liquid chromatography (HPLC)