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毛细管电泳样品电堆积富集过程影响因素的数值分析

曹军,洪芳军,郑平   

  1. School of Mechanical &Power Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
  • 收稿日期:2007-03-09 修回日期:2007-05-21 出版日期:2007-07-30 发布日期:1985-06-25
  • 通讯作者: 郑平

Numerical Analysis on the Influence Factors of Sample Stacking in Capillary Electrophoresis

CAO Jun, HONG Fangjun, CHENG Ping   

  1. School of Mechanical &Power Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
  • Received:2007-03-09 Revised:2007-05-21 Online:2007-07-30 Published:1985-06-25

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摘要： 毛细管电泳样品电堆积富集是一种通过缓冲溶液浓度的差异在毛细管中形成电场强度梯度,从而对样品进行浓缩的富集技术。本文在已有数学模型的基础上,对影响毛细管电堆积富集过程的因素进行了分析。计算结果发现,样品粒子表面所带的电荷电性以及带电量会影响粒子的电泳速度,进而影响富集过程；外加电势的大小会影响样品粒子到达检测窗口的迁移时间；而样品塞的初始长度则会影响样品所能达到的最大富集浓度以及达到最佳的富集效果所需要的时间。所得到的结果对样品电堆积富集技术的进一步完善具有一定的理论指导意义。

关键词: 缓冲溶液, 毛细管电泳, 数值研究 , 样品富集, 影响因素

Abstract:

Sample stacking in capillary electrophoresis can concentrate sample species through the electrical field strength gradient caused by the inhomogeneous buffer concentration field in capillary. The factors that affect the sample stacking process have been analyzed in detail by using a 1-D mathematic model. It was found from the simulation results that the electrical charge number and the electrical charge sign of sample particles can affect the electrophoretic velocity, which in turn has an important influence on the stacking process. The electrical potential can affect the migration time of sample particles to reach detection window, and the initial length of sample plug has significant influence on the maximal sample concentration after stacking and the time to get the optimal stacking effect. The results obtained are helpful to the improvement of the sample stacking technique.

Key words: buffer, influence factors, numerical study , sample stacking, capillary electrophoresis (CE)