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色谱

• 研究论文 • 上一篇    下一篇

毛细管区带电泳法分离白花丹参中主要的水溶性活性成分

崔英杰,李玉琴,史卫峰,杨明峰,赵晓民,夏作理

  

  1. Taishan Medical University, Taian 271000, China

  • 收稿日期:2007-01-26 修回日期:2007-06-06 出版日期:2007-09-30 发布日期:1985-03-25
  • 通讯作者: 崔英杰

Separation of Water-Soluble Active Components in Salvia miltiorrhiza bge. f. alba Using
Capillary Zone Electrophoresis

CUI Yingjie, LI Yuqin, SHI Weifeng, YANG Mingfeng, ZHAO Xiaomin, XIA Zuoli

  

  1. Taishan Medical University, Taian 271000, China

  • Received:2007-01-26 Revised:2007-06-06 Online:2007-09-30 Published:1985-03-25

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摘要：

为建立一种快速分离白花丹参水溶性有效成分的毛细管区带电泳体系,分别考察了缓冲液浓度、缓冲液pH、运行电压、检测波长对样品的分离度、迁移时间等因素的影响。最终优化的分离条件为:5 mmol/L硼砂缓冲液（pH 7.5）;毛细管柱75 μm×60.2 cm,有效长度50 cm,压力进样(3.45 kPa×4 s),27.5 kV恒压分离,210 nm波长下检测,柱温25 ℃。在优化的条件下,8 min内使白花丹参样品中的原儿茶醛、丹参素、原儿茶酸组分达到完全基线分离。

关键词: 白花丹参 , 丹参素, 毛细管区带电泳, 原儿茶醛, 原儿茶酸

Abstract:

To establish a high performance capillary zone electrophoretic method for the separation of water-soluble active components, protocatechuic aldehyde (PAH), danshensu (DSS), and protocatechuic acid (PA), in Salvia miltiorrhiza bge. f. alba, many factors that can affect the resolution and the migration time, including the concentration of phosphate-borax buffer, pH value, separation voltage and detection wavelength were investigated. The optimized conditions were as follows: uncoated capillary column, 75 μm×60.2 cm (effective length of 50 cm); column temperature, 25 ℃; detection wavelength of the photo-diode-array detector, 210 nm; injection, 3.45 kPa×4 s; separation voltage, 27.5 kV; running buffer, 5 mmol/L borax（pH 7.5）. Under the optimized conditions, good separation of PAH, DSS and PA can be achieved in less than 8 min.

Key words: danshensu (DSS), protocatechuic acid (PA), protocatechuic aldehyde (PAH), Salvia miltiorrhiza bge. f. alba , capillary zone electrophoresis (CZE)