色谱

色谱

• 研究论文 • 上一篇    下一篇

高效液相色谱法同时测定化妆品中的7种磺胺及甲硝唑和氯霉素

王萍,李洁,郑和辉

  

  1. Beijing Center for Disease Control and Prevention, Beijing 100013, China

  • 收稿日期:2007-03-22 修回日期:2007-06-12 出版日期:2007-09-30 发布日期:1985-03-25
  • 通讯作者: 王萍

Simultaneous Determination of Seven Sulfonamides and Metronidazole and Chloramphenicol in Cosmetics by High Performance Liquid Chromatography

WANG Ping, LI Jie, ZHENG Hehui

  

  1. Beijing Center for Disease Control and Prevention, Beijing 100013, China

  • Received:2007-03-22 Revised:2007-06-12 Online:2007-09-30 Published:1985-03-25
  • Contact: Wang Ping

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摘要：

建立了化妆品中7种磺胺(磺胺醋酰、磺胺吡啶、磺胺甲基嘧啶、磺胺二甲嘧啶、磺胺甲氧嘧啶、磺胺间甲氧嘧啶、磺胺甲基异唑)和甲硝唑及氯霉素的高效液相色谱测定方法。样品经0.1%甲酸水溶液-乙腈(体积比为8∶2)混合液超声提取后进行液相色谱分析。方法的定量检测限为3～80 μg/g,7种磺胺在20～200 μg/mL时,甲硝唑及氯霉素在40～400 μg/mL时方法的线性关系良好(r≥0.9993)。加标回收率为83.8%～105.3%(7种磺胺的添加水平为50 μg/mL和150 μg/mL，甲硝唑及氯霉素的添加水平为100 μg/mL和300 μg/mL),其相对标准偏差均小于5%。

关键词: 高效液相色谱法, 化妆品 , 磺胺, 甲硝唑, 氯霉素

Abstract:

A method for the simultaneous determination of seven sulfonamides (sulfacetamide, sulfapyridine, sulfamerazine, sulfamethazine, sulfamater, sulfamonomethoxine, sulfamethoxazole)and metronidazole, chloramphenicol in cosmetics has been developed by high performance liquid chromatography with a photodiode array detector(HPLC-PDA). The chromatographic column Atlantis dC18 (150 mm×4.6 mm, 5 μm) was used with acetonitrile-0.1% formic acid (2∶8, v/v) aqueous solution as mobile phase at a flow rate of 1.0 mL/min. Samples were extracted by mobile phase and detected by HPLC-PDA at 268 nm. The limits of quantification were 3-80 μg/g. The correlation coefficients of linear calibration curves were over 0.9993 within the sulfonamide concentration range of 20-200 μg/mL and metronidazole and chloramphenicol concentration range of 40-400 μg/mL. Average recoveries were 83.8%-105.3% at the spiked levels of 50 μg/mL and 150 μg/mL for sulfonamides, and 100 μg/mL and 300 μg/mL for metronidazole and chloramphenicol. The relative standard deviations were below 5%. This method can be routinely used for the determination of seven sulfonamides and metronidazole, chloramphenicol in cosmetics.

Key words: chloramphenicol, cosmetics , metronidazole, sulfonamides, high performance liquid chromatography (HPLC)