色谱

色谱

• 研究论文 • 上一篇    下一篇

高效液相色谱法测定水产品中残留的吡喹酮

沈晓盛,于慧娟,蔡友琼,黄冬梅

  

  1. The Fishery Products Quality Inspection and Test Center of Ministry of Agriculture, East China Sea Fishery Research Institute, Chinese Academy of Fishery Science, Shanghai 200090, China
  • 收稿日期:2007-04-20 修回日期:2007-07-02 出版日期:2007-11-30 发布日期:1984-12-25
  • 通讯作者: 于慧娟

Determination of Praziquantel Residue in Aquatic Products Using High Performance Liquid Chromatography

SHEN Xiaosheng, YU Huijuan, CAI Youqiong, HUANG Dongmei   

  1. The Fishery Products Quality Inspection and Test Center of Ministry of Agriculture, East China Sea Fishery Research Institute, Chinese Academy of Fishery Science, Shanghai 200090, China
  • Received:2007-04-20 Revised:2007-07-02 Online:2007-11-30 Published:1984-12-25
  • Contact: YU Huijuan

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摘要：

建立了一种灵敏度高、操作简单的定量分析水产品中残留的微量吡喹酮的高效液相色谱方法。样品经乙酸乙酯提取、极性硅胶柱净化后进行高效液相色谱分析,以乙腈-水(体积比为50∶50)为流动相,流速为0.9 mL/min,ZORBAX C18色谱柱分离,紫外检测波长为214 nm。在此条件下吡喹酮在0.02～20 mg/L范围内呈线性关系,相关系数为0.99998；吡喹酮的加标回收率在85%以上,检出限为10 μg/kg。

关键词: 吡喹酮残留, 高效液相色谱法, 水产品

Abstract:

A sensitive and simple method for the determination of praziquantel residue in aquatic products has been established. The sample was extracted with ethyl acetate for three times (20 mL, 20 mL, 10 mL) and cleaned up with an LC-Si column. The extract was separated on a ZORBAX SB-C18 column (250 mm×4.6 mm, 5 μm). Ultraviolet detection was performed at 214 nm. Acetonitrile-water (50∶50, v/v) was used as the mobile phase at a flow rate of 0.9 mL/min. The calibration curve of praziquantel in aquatic products at the concentration range of 0.02-20 mg/L was linear （r=0.99998）. The recovery of praziquantel was higher than 85%. The limit of detection was 10 μg/kg （S/N＞3）.

Key words: aquatic products , praziquantel residue, high performance liquid chromatography （HPLC）