色谱

色谱

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高效液相色谱法同时测定罗汉果中的六种葫芦烷三萜类皂苷

卢凤来,刘金磊,黄永林,李典鹏   

  1. Guangxi Institute of Botany, the Chinese Academy of Sciences, Guilin 541006, China
  • 收稿日期:2008-07-30 修回日期:2008-07-30 出版日期:2008-07-30 发布日期:1983-12-25
  • 通讯作者: 李典鹏
  • 基金资助:
    中国科学院“西部之光”人才培养计划入选项目(科发人教字［2002］101号)和桂林市科技经费资助项目(编号:20070304-2).

Simultaneous determination of six cucurbitane triterpene glycosides in Siraitia grosvenorii fruits using high performance liquid chromatography

LU Fenglai, LIU Jinlei, HUANG Yonglin, LI Dianpeng   

  1. Guangxi Institute of Botany, the Chinese Academy of Sciences, Guilin 541006, China
  • Received:2008-07-30 Revised:2008-07-30 Online:2008-07-30 Published:1983-12-25
  • Contact: LI Dianpeng

PDF

705

被引次数

15

摘要： 建立了高效液相色谱测定罗汉果中罗汉果苷Ⅴ、罗汉果苷ⅣA、罗汉果苷Ⅲ、11-氧化罗汉果苷Ⅲ、罗汉果苷ⅡE和11-氧化罗汉果苷ⅡE等6种苷类化合物含量的方法。采用的色谱条件：ZORBAX SB-C18色谱柱(150 mm×4.6 mm, 5 μm);柱温30 ℃;以水(A相)和乙腈(B相)为流动相,梯度洗脱程序：0～3 min，20%B～30%B;3～8 min,30%B～35%B;8～9 min,35%B;流速0.8 mL/min;检测波长203 nm;进样量10 μL。结果表明,以上6种苷类化合物分别在0.04～1.0 mg/mL、0.011～0.68 mg/mL、0.010～0.80 mg/mL、0.0097～0.58 mg/mL、0.025～1.0 mg/mL、0.013～0.76 mg/mL范围内线性关系良好(r＞0.9991)。加标回收率依次为99.65%,101.6%,97.05%,103.1%,99.25%和103.0%;相对标准偏差分别为0.83%，3.1%，1.9%，3.3%，0.59%和2.0%。该方法简便、快速、准确,适用于罗汉果原材料及其制品中苷类化合物的检测和含量测定。

关键词: 高效液相色谱法, 葫芦烷三萜类皂苷, 罗汉果

Abstract: Siraitia grosvenorii, a traditional Chinese fruit, belongs to the family Cucurbitaceae and has been used as a pulmonary demulcent and emollient for the treatment of dry cough, sore throat, dire thirst, and constipation in folk medicine. A high performance liquid chromatographic method was developed for simultaneous determining the contents of mogrosideⅤ, mogrosideⅣA, mogrosideⅢ, 11-oxomogrosideⅢ, mogrosideⅡE and 11-oxomogrosideⅡE in Siraitia grosvenorii fruits. The chromatographic analysis was carried out on a ZORBAX SB-C18 column (150 mm×4.6 mm, 5 μm). The mobile phase was water (A) and acetonitrile (B) with gradient elution (0－3 min, 20%B-30%B; 3-8 min, 30%B-35%B; 8-9 min, 35%B). The flow rate was maintained at 0.8 mL/min. The detection wavelength was set at 203 nm and the column temperature was controlled at 30 ℃. The sample injection volume was 10 μL. The calibration curves were linear over the ranges of 0.04-1.0 mg/mL, 0.011-0.68 mg/mL, 0.010-0.80 mg/mL, 0.0097-0.58 mg/mL, 0.025-1.0 mg/mL and 0.013-0.76 mg/mL (r>0.9991) for the above cucurbitane triterpene glycosides, respectively. The average recoveries were 99.65% for mogrosideⅤ, 101.6% for mogroside ⅣA, 97.05% for mogroside Ⅲ, 103.1% for 11-oxomogrosideⅢ, 99.25% for mogrosideⅡE, and 103.0% for 11-oxomogrosideⅡE, with the relative standard deviations of 0.83%, 3.1%, 1.9%, 3.3%, 0.59% and 2.0%, respectively. This simple, rapid and accurate method is suitable for quality control and determination of raw materials and products of Siraitia grosvenorii fruits.

Key words: cucurbitane triterpene glycosides, Siraitia grosvenorii , high performance liquid chromatography (HPLC)