色谱

色谱

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高效液相色谱法同时测定厚朴温中胶囊中的7种有效成分

丁晓菊,赵云丽,高晓霞,唐倩,李琳,于治国*   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • 收稿日期:2008-07-23 修回日期:2008-09-23 出版日期:2009-01-30 发布日期:1983-03-25
  • 通讯作者: 于治国
  • 基金资助:

Simultaneous determination of seven effective components in Houpuwenzhong capsules using high performance liquid chromatography

DING Xiaoju, ZHAO Yunli, GAO Xiaoxia, TANG Qian, LI Lin, YU Zhiguo*   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2008-07-23 Revised:2008-09-23 Online:2009-01-30 Published:1983-03-25
  • Contact: YU Zhiguo

PDF

524

被引次数

25

摘要： 建立了同时测定厚朴温中胶囊中山姜素、甘草酸、和厚朴酚、小豆蔻明、木香烃内酯、去氢木香内酯及厚朴酚含量的反相高效液相色谱法。固定相为Scienhome C18柱(250 mm×4.6 mm,5 μm),流动相为甲醇-乙腈-0.06%磷酸溶液(体积比为38∶27∶35),流速为1.0 mL/min,柱温为30 ℃,检测波长为235 nm。在上述条件下,山姜素、甘草酸、和厚朴酚、小豆蔻明、木香烃内酯、去氢木香内酯及厚朴酚的质量浓度分别在0.885～17.7,107～2140,8.85～17.7,1.035～20.7,4.85～97,5.9～118和17.5～350 mg/L时与色谱峰面积之间的线性关系良好;回收率分别为96.9%～101.1%,96.0%～100.5%,100.3%～100.8%,97.7%～101.4%,100.4%～102.3%,96.0%～102.3%和96.2%～100.6%。该方法简便、快速、准确,可用于厚朴温中胶囊的质量控制。

关键词: 高效液相色谱法, 厚朴温中胶囊 , 有效成分

Abstract: A high performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of seven effective components in Houpuwenzhong capsules. The separation was performed on a Scienhome C18 column (250 mm×4.6 mm, 5 μm) with methanol-acetonitrile-0.06% phosphonic acid (38∶27∶35, v/v/v) as the mobile phase at a flow rate of 1.0 mL/min and 30 ℃. The detective wavelength was set at 235 nm. There were good linear relationships between the mass concentrations and the peak areas of alpinetin, glycyrrhizic acid, honokiol, cardamonin, costunolide, dehydrocostus lactone and magnolol in the ranges of 0.885-17.7, 107-2140, 8.85-17.7, 1.035-20.7, 4.85-97, 5.9-118 and 17.5-350 mg/L, respectively. The recoveries were 96.9%-101.1%, 96.0%-100.5%, 100.3%-100.8%, 97.7%-101.4%, 100.4%-102.3%, 96.0%-102.3% and 96.2%-100.6%, respectively. This method is rapid, simple and suitable for the quality control of Houpuwenzhong capsules.

Key words: effective components, Houpuwenzhong capsules , high performance liquid chromatography (HPLC)