色谱

色谱

• 研究论文 • 上一篇    下一篇

同位素稀释液相色谱-串联质谱法测定化妆品中的N-亚硝基二乙醇胺

马强*, 王超, 白桦, 王星, 董益阳, 武婷, 张庆, 王军兵, 唐英章   

  1. Chinese Academy of Inspection and Quarantine, Beijing 100123, China
  • 收稿日期:2008-10-28 修回日期:2008-12-29 出版日期:2009-05-30 发布日期:1982-09-25
  • 通讯作者: 马强

Determination of N-nitrosodiethanolamine in cosmetics by isotope dilution-liquid chromatography- tandem mass spectrometry

MA Qiang*, WANG Chao, BAI Hua, WANG Xing, DONG Yiyang, WU Ting, ZHANG Qing, WANG Junbing, TANG Yingzhang   

  1. Chinese Academy of Inspection and Quarantine, Beijing 100123, China
  • Received:2008-10-28 Revised:2008-12-29 Online:2009-05-30 Published:1982-09-25
  • Contact: MA Qiang

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822

被引次数

11

摘要： 建立了化妆品中N-亚硝基二乙醇胺(NDELA)的同位素稀释液相色谱-串联质谱分析方法。水溶性化妆品样品以水为提取溶剂提取,样品提取液经高速离心处理后,上清液过Oasis HLB固相萃取柱净化。脂溶性化妆品样品用二氯甲烷和水混合溶剂进行液-液分配萃取。NDELA经Waters Atlantis T3色谱柱(150 mm×2.1 mm,3 μm)分离后在多反应监测模式下进行串联质谱定性及定量分析,以d8-NDELA为内标定量。NDELA的方法定量限为50 μg/kg;在50-250 μg/kg的3个添加水平范围内的平均回收率为89.1%～98.2%,日内精密度均小于9%,日间精密度均小于11%。该方法能够满足化妆品中NDELA的检测要求。

关键词: N-亚硝基二乙醇胺, 化妆品 , 同位素稀释, 液相色谱-串联质谱

Abstract: A comprehensive analytical method based on isotope dilution-liquid chromatography-tandem mass spectrometry has been developed for the determination of N-nitrosodiethanolamine in cosmetics. Water-soluble cosmetic samples were extracted with water. The extract was centrifuged, then the upper solution was cleaned up by an Oasis HLB solid phase extraction cartridge. Oil-soluble cosmetic samples were extracted by liquid-liquid partition with dichloromethane and water. Qualitative and quantitative analyses were carried out for the analyte under the multiple reaction monitoring (MRM) mode after the chromatographic separation on a Waters Atlantis T3 column (150 mm×2.1 mm, 3 μm ). The quantitation was performed with deuterated N-nitrosodiethanolamine as internal standard. The limit of quantitation (LOQ) for N-nitrosodiethanolamine was 50 μg/kg. The mean recoveries were 89.1%~98.2% at the spiked levels of 50~250 μg/kg, with the intra-day precision less than 9% and the inter-day precision less than 11%. The method is suitable for the determination of NDELA in cosmetics.

Key words: cosmetics , liquid chromatography-tandem mass spectrometry, N-nitrosodiethanolamine, isotope dilution