色谱

色谱

• 研究论文 • 上一篇    下一篇

基于三波长融合谱的系统指纹定量法鉴定龙胆泻肝丸的真实质量

孙国祥*, 张静娴   

  1. College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • 收稿日期:2008-11-07 修回日期:2009-01-07 出版日期:2009-05-30 发布日期:1982-09-25
  • 通讯作者: 孙国祥

Identification of the authentic quality of Longdanxiegan pill by systematic quantified fingerprint method based on three wavelength fusion chromatogram

SUN Guoxiang*, ZHANG Jingxian   

  1. College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2008-11-07 Revised:2009-01-07 Online:2009-05-30 Published:1982-09-25
  • Contact: SUN Guoxiang

PDF

766

被引次数

66

摘要： 建立了龙胆泻肝丸(Longdanxiegan pill,LDXGP)三波长融合高效液相色谱(HPLC)指纹图谱,以系统指纹定量法全面鉴定LDXGP的质量。采用反相高效液相色谱法(RP-HPLC),运用多波长融合指纹图谱技术对色谱图进行处理,以黄芩苷为参照物峰,确立了63个共有指纹峰,以宏定性相似度为参量对12个厂家的12批LDXGP进行聚类分析,确定用其中10批生成对照指纹图谱(RFP),以此RFP为标准用系统指纹定量法评价12批LDXGP的质量。结果鉴别出9批质量完全合格,1批含量明显偏高,2批化学成分数量和分布比例不合格。基于多波长融合技术的系统指纹定量法是评价中药真实质量的可靠方法。

关键词: 高效液相色谱法, 宏定量相似度, 宏定性相似度, 龙胆泻肝丸 , 三波长融合指纹图谱, 系统指纹定量法

Abstract: The three wavelength fusion high performance liquid chromatographic fingerprints (TWFFP) of Longdanxiegan pill (LDXGP) was established to identify the quality of LDXGP by the systematic quantified fingerprint method. The chromatographic fingerprints (CFPs) of the 12 batches of LDXGP were determined by reversed-phase high performance liquid chromatography. The technique of multi-wavelength fusion fingerprint was applied during processing the fingerprints. The TWFFPs containing 63 co-possessing peaks were obtained when choosing baicalin peak as the referential peak. The 12 batches of LDXGP were identified with hierarchical clustering analysis by using macro qualitative similarity (Sm) as the variable. According to the results of classification, the referential fingerprint (RFP) was synthesized from 10 batches of LDXGP. Taking the RFP for the qualified model, all the 12 batches of LDXGP were evaluated by the systematic quantified fingerprint method. Among the 12 batches of LDXGP, 9 batches were completely qualified, the contents of 1 batch were obviously higher while the chemical constituents quantity and distributed proportion in 2 batches were not qualified. The systematic quantified fingerprint method based on the technique of multi-wavelength fusion fingerprint can effectively identify the authentic quality of traditional Chinese medicine.

Key words: Longdanxiegan pill (LDXGP) , macro qualitative similarity, macro quantitative similarity, systematic quantified fingerprint method, three wavelength fusion fingerprint, high performance liquid chromatography (HPLC)