色谱

色谱

• 研究论文 • 上一篇    下一篇

固相萃取净化-正相高效液相色谱测定纺织品中残留的烷基酚和烷基酚聚氧乙烯醚

吕春华*, 陈笑梅, 刘海山   

  1. Zhejiang Entry-Exit Inspection and Quarantine Bureau, Hangzhou 310012, China
  • 收稿日期:2009-02-05 修回日期:2009-04-03 出版日期:2009-07-30 发布日期:1982-06-25
  • 通讯作者: 吕春华

Determination of alkylphenols and alkylphenol polyethoxylates in textiles by normal-phase high performance liquid chromatography and solid-phase extraction

Lv Chunhua*, CHEN Xiaomei, LIU Haishan   

  1. Zhejiang Entry-Exit Inspection and Quarantine Bureau, Hangzhou 310012, China
  • Received:2009-02-05 Revised:2009-04-03 Online:2009-07-30 Published:1982-06-25
  • Contact: Lv Chunhua

PDF

771

被引次数

23

摘要： 建立了测定纺织品中烷基酚(AP)和烷基酚聚氧乙烯醚(APnEO)(n2～16)的正相高效液相色谱法。以甲醇为提取溶剂,用索氏抽提法提取纺织品中的AP和APnEO,用正相高效液相色谱法测定,并对提取溶剂、提取方式和净化条件等前处理条件进行优化。该方法的检出限为1.0 mg/kg,回收率为90.4%～104.1%,相对标准偏差为0.64%～4.21%。采用该方法对多种基质纺织品进行检测,可满足残留检测要求。

关键词: 纺织品 , 烷基酚, 烷基酚聚氧乙烯醚, 正相高效液相色谱法

Abstract: A method for the simultaneous determination of alkylphenols (AP) and alkylphenol polyethoxylates (APnEO)(n2~16) in textiles was developed by normal-phase high performance liquid chromatography (NP-HPLC). The residues of AP and APnEO in the samples were extracted with methanol by Soxhlet extraction. The pretreatment conditions such as extraction solvents, extraction methods and clean-up methods were optimized. The sample was purified by an HLB solid-phase extraction column and separated on an amino column (250 mm×4.6 mm, 5 μm) with hexane-isopropanol (90:10, v/v) and isopropanol-water (90:10, v/v) as mobile phases at a flow rate of 1.0 mL/min and determined by a fluorescence detector. AP and each APnEO oligomer were separated successfully within a reasonable time with gradient elution, which are difficult to be separated by using conventional reversed-phase high performance liquid chromatography (RP-HPLC). The detection limit was 1.0 mg/kg for each analyte. The recoveries ranged from 90.4% to 104.1% with the relative standard deviations (RSDs) ranged from 0.64% to 4.21%. This method is suitable for the determination of alkylphenols and alkylphenol polyethoxylates in textiles.

Key words: alkylphenol polyethoxylates, alkylphenols, textiles , normal-phase high performance liquid chromatography (NP-HPLC)