色谱

色谱

• 技术与应用 • 上一篇    下一篇

中药滴水珠中Neoechinulin A的分离及测定

王琦, 赵云丽, 高晓霞, 王欣杨, 于治国*   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • 收稿日期:2008-11-20 修回日期:2009-01-20 出版日期:2009-07-30 发布日期:1982-06-25
  • 通讯作者: 于治国

Isolation and determination of Neoechinulin A in Cordate Pinellia Tuber

WANG Qi, ZHAO Yunli, GAO Xiaoxia, WANG Xinyang, YU Zhiguo*   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2008-11-20 Revised:2009-01-20 Online:2009-07-30 Published:1982-06-25
  • Contact: YU Zhiguo

PDF

643

被引次数

13

摘要： 采用柱色谱法对中药滴水珠中的有效成分Neoechinulin A进行分离纯化,运用现代波谱技术进行结构鉴定,采用反相高效液相色谱法测定其含量。色谱条件: Diamonsil C18柱(250 mm×4.6 mm,5 μm),流动相为甲醇-0.1%磷酸溶液(体积比为63:37),流速为1.0 mL/min,柱温为30 ℃,检测波长为225 nm,进样量为10 μL。结果表明,Neoechinulin A的质量浓度在2.0～40.0 mg/L范围内与其峰面积有良好的线性关系(r0.9995)；方法的加样回收率为98.3%～101.1%。该方法简便、快速、准确,可作为中药滴水珠质量控制的一个有效方法。

关键词: Neoechinulin A, 滴水珠, 反相高效液相色谱法, 吲哚类生物碱, 中药

Abstract: The Neoechinulin A in Cordate Pinellia Tuber was isolated by column chromatography and identified by mass spectrometry and nuclear magnetic resonance (NMR). A method of reversed-phase high performance liquid chromatography (RP-HPLC) for the determination of the Neoechinulin A in Cordate Pinellia Tuber was developed. The chromatography was performed on a Diamonsil C18 column (250 mm×4.6 mm, 5 μm) with a mixture of methanol and 0.1% phosphoric acid solution (63:37, v/v) as mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was set at 225 nm and the column oven temperature was set at 30 ℃. The volume of injection was 10 μL. There was a good linear relationship (r0.9995) between the mass concentration and the peak area of Neoechinulin A in the range of 2.0~40.0 mg/L. The recovery was 98.3%~101.1%. The method is rapid and simple with good accuracy, reproducibility and suitable for the quality control of Cordate Pinellia Tuber from different sources.

Key words: Chinese herbal drug , Cordate Pinellia Tuber, indole alkaloids, Neoechinulin A, reversed-phase high performance liquid chromatography (RP-HPLC)