色谱

色谱

• 技术与应用 • 上一篇    下一篇

离子色谱法同时测定水源水中的5种生物胺

赵新颖1*, 焦霞2, 夏敏1, 刘清1, 张经华1   

  1. 1.Beijing Center for Physical and Chemical Analysis, Beijing 100089, China; 2.Qingdao Shenghan Chromatography Tech Equipment Co, Ltd., Qingdao 266101, China
  • 收稿日期:2009-02-22 修回日期:2009-04-22 出版日期:2009-07-30 发布日期:1982-06-25
  • 通讯作者: 赵新颖

Determination of five biogenic amines in source water by ion chromatography

ZHAO Xinying1*, JIAO Xia2, XIA Min1, LIU Qing1, ZHANG Jinghua1   

  1. 1.Beijing Center for Physical and Chemical Analysis, Beijing 100089, China; 2.Qingdao Shenghan Chromatography Tech Equipment Co, Ltd., Qingdao 266101, China
  • Received:2009-02-22 Revised:2009-04-22 Online:2009-07-30 Published:1982-06-25
  • Contact: ZHAO Xinying

PDF

930

被引次数

37

摘要： 建立了离子色谱(IC)同时测定水源水中5种生物胺(BA)(腐胺、尸胺、组胺、亚精胺和精胺)的方法。样品经0.45 μm水膜过滤后,用TSK-GEL SuperIC阳离子交换柱(150 mm×4.6 mm)分离,以甲磺酸水溶液为流动相梯度淋洗,流速为1.0 mL/min,非抑制电导检测,进样量为100 μL。实验结果表明,5种生物胺可以实现基线分离;在1.0～30.0 mg/L范围内,其峰面积与质量浓度之间的线性关系良好;保留时间的相对标准偏差(RSD)均不高于0.02%,峰面积的RSD小于2.08%;加标回收率为96.0%～107.0%。该法简便、快捷、准确,可以用于水源水中腐胺、尸胺、组胺、亚精胺和精胺5种生物胺的同时测定。

关键词: 腐胺, 精胺, 离子色谱法, 生物胺, 尸胺, 水源水 , 亚精胺, 组胺

Abstract: An ion chromatographic (IC) method was developed for the separation of five biogenic amines (BAs), including putrescine, cadaverine, histamine, spermidine and spermine, in source water. The sample was separated on a TSK-GEL SuperIC cation exchange column (150 mm×4.6 mm) after filtering treatment by 0.45 μm inorganic membrane. Methanesulfonic acid was used as eluent for concentration gradient elution at the rate of 1.0 mL/min. The injection volume was 100 μL and the BAs were detected by a non-suppressed conductivity detector. The result showed that the baseline separation was obtained for the 5 kinds of BAs. The linear relationships between the concentration and the peak area were obtained in the concentration range of 1.0~30.0 mg/L. The relative standard deviations (RSDs) of retention time and peak area were no more than 0.02% and 2.08%, respectively. The recoveries were from 96.0% to 107.0%. The method is simple, rapid, highly sensitive and with good repeatability. It could be used for the determination the BAs in the source water.

Key words: biogenic amines (BAs), cadaverine, histamine, putrescine, source water , spermidine, spermine, ion chromatography (IC)