色谱

色谱 ›› 2012, Vol. 30 ›› Issue (08): 770-776.DOI: 10.3724/SP.J.1123.2012.04001

• 研究论文 • 上一篇    下一篇

超快速液相色谱-离子阱飞行时间质谱法测定肉制品中10种碱性染料

张东雷1*, 汪丽娜2, 陈小珍1, 王瑾1, 曹慧1, 黄丽英1   

  1. 1. 浙江省质量检测科学研究院, 浙江 杭州 310013; 2. 浙江工业大学化学工程与材料学院, 浙江 杭州 310014
  • 收稿日期:2012-04-05 修回日期:2012-06-06 出版日期:2012-08-28 发布日期:2012-08-17
  • 通讯作者: 张东雷,高级工程师,主要从事食品安全检测及实验室管理工作. E-mail: fyzdl@163.com.
  • 基金资助:

    浙江省质量技术监督系统科研计划项目(20100104)和浙江省公益性技术应用研究(分析测试)项目(2011C37048).

Determination of ten basic dyes in meat products by ultra fast liquid chromatography-ion trap time of flight mass spectrometry

ZHANG Donglei1*, WANG Lina2, CHEN Xiaozhen1, WANG Jin1, CAO Hui1, HUANG Liying1   

  1. 1. Zhejiang Institute of Quality Inspection Science, Hangzhou 310013, China; 2. College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310014, China
  • Received:2012-04-05 Revised:2012-06-06 Online:2012-08-28 Published:2012-08-17

PDF

571

被引次数

28

摘要： 建立了肉制品中10种碱性染料的超快速液相色谱-离子阱飞行时间质谱(LC-IT-TOF-MS)检测方法。使用Waters AcquityTM UPLC BEH C18(100 mm×2.1 mm,1.7 μm)作为分析柱,以5 mmol/L乙酸铵水溶液-含0.1%(体积分数)甲酸的乙腈溶液为流动相进行梯度洗脱,流速0.2 mL/min。采用电喷雾离子化源,在正离子模式下进行检测。样品用乙腈提取,经弱阳离子交换(Oasis WCX)固相萃取柱净化,超快速液相色谱分离,外标法定量。结果表明,10种碱性染料在1.0～100.0 μg/L范围内线性关系良好,r2均大于0.9915,相对标准偏差(n=7)均小于8.54%。在2,10,25 μg/kg 3个添加水平下平均回收率为65.39%～119.18%。该方法简单、灵敏度高、分析时间短,适用于肉制品中多种碱性染料的同时测定。

关键词: 超快速液相色谱-离子阱飞行时间质谱, 碱性染料, 肉制品

Abstract: A method was developed for the simultaneous determination of 10 basic dyes in meat products using ultra fast liquid chromatography-ion trap time of flight mass spectrometry (LC-IT-TOF-MS). The target analytes were separated at a flow rate of 0.2 mL/min on a Waters AcquityTM UPLC BEH C18 (100 mm×2.1 mm, 1.7 μm) column with a gradient elution. The mobile phase was 5 mmol/L ammonium acetate-acetonitrile (containing 0.1%(v/v) formic acid). The identification and quantification were achieved in positive ion mode with electro spray ionization source. The samples were extracted with a simple procedure using acetonitrile and cleaned up by weak cation exchange (Oasis WCX)solid phase extraction column. Ten basic dyes were determined by LC-IT-TOF-MS, and quantified by external standard method. The developed method showed a good linearity over the wide range of 1.0~100.0 μg/L, and the relative standard deviations (n=7) were less than 8.54%. The average recoveries of the ten basic dyes at three levels (2, 10 and 25 μg/kg) were ranged from 65.39% to 119.18%. Therefore, this method, owing to its simplicity, rapidity and high sensitivity, has a good applicability to the simultaneous determination of dye residues in meat products.

Key words: basic dyes, meat products, ultra fast liquid chromatography-ion trap time of flight mass spectrometry (LC-IT-TOF-MS)