色谱

色谱 ›› 2012, Vol. 30 ›› Issue (09): 938-942.DOI: 10.3724/SP.J.1123.2012.04025

• 研究论文 • 上一篇    下一篇

毛细管电泳电致化学发光法同时测定氯丙嗪、异丙嗪及其主要代谢物

李旭菲, 杨燕英, 周考文*   

  1. 北京联合大学生物化学工程学院, 北京 100023
  • 收稿日期:2012-04-17 修回日期:2012-07-17 出版日期:2012-09-28 发布日期:2012-09-20
  • 通讯作者: 周考文,教授,研究方向为化学发光分析. Tel: (010)52072102, E-mail: zhoukaowen@gmail.com.
  • 基金资助:

    北京市教委科技发展计划(KM200711417005)和北京市大学生科技创新项目(2012L0059).

Simultaneous determination of chlorpromazine and promethazine and their main metabolites by capillary electrophoresis with electrochemiluminescence

LI Xufei, YANG Yanying, ZHOU Kaowen*   

  1. Biochemical Engineering College, Beijing Union University, Beijing 100023, China
  • Received:2012-04-17 Revised:2012-07-17 Online:2012-09-28 Published:2012-09-20

PDF

397

被引次数

19

摘要： 建立了同时检测氯丙嗪、异丙嗪及其主要代谢物的毛细管电泳电致化学发光新方法。最佳实验条件为: 检测电位1.20 V,钌联吡啶浓度5 mmol/L,检测池磷酸缓冲溶液40 mmol/L (pH 6.5),分离磷酸缓冲溶液18 mmol/L (pH 4.8),进样电压11 kV,进样时间8 s,分离电压13.5 kV。方法的检出限(3σ)分别为氯丙嗪8.3×10~7 g/L、异丙嗪7.2×10~6 g/L、氯丙嗪亚砜1.9×10~5g/L和异丙嗪亚砜3.7×10~6g/L,各组分的电化学发光强度和迁移时间的相对标准偏差(RSD)分别不超过3%和1%。本方法具有简便、快速、灵敏、进样量少和不受共存物干扰等特点,可在不必预分离的情况下直接同时连续测定家犬尿样中的氯丙嗪、异丙嗪、氯丙嗪亚砜和异丙嗪亚砜。

关键词: 电致化学发光, 氯丙嗪, 氯丙嗪亚砜, 毛细管电泳, 尿样, 异丙嗪, 异丙嗪亚砜

Abstract: Based on the phenomenon that each of chlorpromazine (CPZ), promethazine (PMZ), chlorpromazine sulfoxide (CPZSO) and promethazine sulfoxide (PMZSO) could enhance the electrochemiluminescence (ECL) intensity of tris(2,2′-bipyridyl) ruthenium, a novel and sensitive method was proposed for the simultaneous determination of CPZ, PMZ and their main metabolites using capillary electrophoresis (CE) coupled with ECL detection. The influences of several experimental parameters were explored. The optimum experimental conditions were as follows: detection potential of 1.20 V (Ag/AgCl), 40 mmol/L of phosphate buffer solution (pH 6.5) containing 5 mmol/L tris(2,2′-bipyridyl) ruthenium in ECL detection cell, running buffer solution of 18 mmol/L (pH 4.8), sample injection of 8 s at 11 kV, and separation voltage of 13.5 kV. The detection limits (3σ) of this method were 8.3×10~7g/L for CPZ, 7.2×10~6g/L for PMZ, 1.9×10~5g/L for CPZSO and 3.7×10~6g/L for PMZSO. The linear ranges of ECL intensity versus mass concentration of medicaments were 7.1×10~6~6.3×10~3g/L for CPZ, 7.5×10~5~4.6×10~3g/L for PMZ, 9.7×10~5~3.6×10~3g/L for CPZSO and 8.1×10~5~7.7×10~3g/L for PMZSO. The relative standard deviations (RSDs) of the four target compounds were not more than 3% for ECL intensity and 1% for migration time. This method has the merits of simplicity, speediness, sensitivity, small sample injection, and free from interference. This method was successfully utilized to directly and simultaneously detect CPZ, PMZ, CPZSO and PMZSO in urine samples of pet dogs.

Key words: chlorpromazine, chlorpromazine sulfoxide, electrochemiluminescence, promethazine, promethazine sulfoxide, urine sample, capillary electrophoresis (CE)