色谱

色谱 ›› 2015, Vol. 33 ›› Issue (2): 174-181.DOI: 10.3724/SP.J.1123.2014.09042

• 技术与应用 • 上一篇    下一篇

顶空固相微萃取-全二维气相色谱/飞行时间质谱法分析闽南乌龙茶中的挥发性成分及其在分类中的应用

程权2, 杨方1, 李捷1, 卢声宇1, 蓝锦昌1, 江锦彬1   

  1. 1. 福建出入境检验检疫局检验检疫技术中心, 福建省检验检疫重点实验室, 福建 福州 350001;
    2. 食品安全 分析与检测教育部重点实验室, 福建省食品安全分析与检测重点实验室, 福州大学化学系, 福建 福州 350108
  • 收稿日期:2014-09-25 修回日期:2014-10-30 出版日期:2015-02-08 发布日期:2015-01-24
  • 通讯作者: 潘再法
  • 基金资助:

    国家质检总局科研项目(2013IK147);福建省科技重点项目(2012Y6001).

Analysis of the volatile components in Minnan oolong tea by headspace solid phase microextraction coupled with comprehensive two-dimensional gas chromatography- time of flight mass spectrometry and the application in its variety identification

CHENG Quan2, YANG Fang1, LI Jie1, LU Shengyu1, LAN Jinchang1, JIANG Jinbin1   

  1. 1. Inspection and Quarantine Technical Center of Fujian Entry-Exit Inspection & Quarantine Bureau, Key Laboratory of Inspection and Quarantine of Fujian, Fuzhou 350001, China;
    2. Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry of Fuzhou University, Fuzhou 350108, China
  • Received:2014-09-25 Revised:2014-10-30 Online:2015-02-08 Published:2015-01-24

PDF

474

被引次数

16

摘要：

采用顶空固相微萃取(HS-SPME)结合全二维气相色谱/飞行时间质谱(GC×GC-TOF MS)分析了闽南乌龙茶中的挥发性成分。从48份不同等级和产季的乌龙茶(铁观音、黄金桂、本山、毛蟹和梅占)中获得了2000余种挥发性化合物,经筛选得到51种共有组分,并结合质谱数据库、保留指数与结构谱图等进行了初步鉴定。在此基础上采用主成分分析法(PCA)获得得分投影图,直观给出了不同样品的分类趋势。通过逐步判别获得9种对分类结果有显著影响的组分,并以此为变量通过Fisher判别法(FDA)建立了4个判别函数,对样品的分类准确率达到97.9%。本试验证实了以挥发性成分识别闽南乌龙茶的可行性。

关键词: Fisher判别分析, 顶空固相微萃取, 挥发性成分, 闽南乌龙茶, 全二维气相色谱/飞行时间质谱, 主成分分析

Abstract:

A method to analyze the volatile components in Minnan oolong tea was developed based on headspace solid phase microextraction (HS-SPME) coupled with comprehensive two-dimensional gas chromatography-time of flight mass spectrometry (GC×GC-TOF MS). Volatile compounds of 48 oolong tea samples with different qualities and production seasons from five varieties (Tieguanyin, Huangjingui, Benshan, Maoxie and Meizhan) were extracted by HS-SPME and analyzed by GC×GC-TOF MS. More than 2000 peaks were obtained from each sample, and 51 common compounds were tentatively identified by comparison with the standard mass spectrum databases, retention indices and structure spectra. The projection score of the common compounds obtained from principal component analysis (PCA) had presented a straightforward classification trend for different oolong tea varieties. In addition, 9 compounds which had significant impact on the classification were selected by stepwise discriminate analysis, and used as variables to establish four discriminated functions by Fisher's discriminate analysis (FDA). The accuracy for the recognition of 48 samples was 97.9%. The results had demonstrated the feasibility of the method to be used to discriminate the oolong tea varieties.

Key words: comprehensive two-dimensional gas chromatography-time of flight mass spectrometry (GC×GC-TOF MS), Fisher's discriminate analysis, headspace solid phase microextraction (HS-SPME), Minnan oolong tea, principal component analysis (PCA), volatile components

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