色谱

色谱 ›› 2016, Vol. 34 ›› Issue (8): 778-782.DOI: 10.3724/SP.J.1123.2016.04006

• 研究论文 • 上一篇    下一篇

顶空固相微萃取-气相色谱-质谱联用分析费约果叶片挥发性香气成分条件优化

白俊英, 黄仁华, 陆云梅, 余游, 王丹   

  1. 西南科技大学生命科学与工程学院, 四川 绵阳 621010
  • 收稿日期:2016-04-05 出版日期:2016-08-08 发布日期:2016-07-26
  • 通讯作者: 黄仁华
  • 基金资助:

    国家自然科学基金项目（31000259）；四川省科技厅"十一五"科技项目（14zs2105）；校实验室开放基金项目（15xnkf03）.

Optimization of headspace solid phase micro-extraction combined with gas chromatography-mass spectrometry conditions of volatile flavor from Feijoa sellowiana leaves

BAI Junying, HUANG Renhua, LU Yunmei, YU You, WANG Dan   

  1. College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
  • Received:2016-04-05 Online:2016-08-08 Published:2016-07-26
  • Supported by:

    Natural Science Foundation of China (No. 31000259); "11th Five-Year" Science and Technology Project of Sichuan Provincial Science and Technology Department (No. 14zs2105); the Open Fund of School Laboratory (No. 15xnkf03).

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被引次数

4

摘要：

为了建立适合费约果叶片香气成分检测的顶空固相微萃取-气相色谱-质谱（HS-SPME-GC-MS）分析方法，考察HS-SPME中不同萃取条件对香气萃取效果的影响，采用L9（34）正交试验，以出峰个数和总峰面积为考察指标确定最佳萃取条件。结果表明，萃取温度对出峰个数和总峰面积的影响较大，而加样量的影响较小。实验得出的较优萃取条件为：加样量0.6 g，萃取温度50 ℃，萃取时间50 min，解吸时间6 min。在此萃取条件下能够得到88种物质，这些物质能更真实地反映费约果叶片香气成分的化学构成，为费约果叶片的开发利用提供有价值的数据。

关键词: 顶空固相微萃取, 费约果叶片, 挥发性香气成分, 气相色谱-质谱, 正交试验, 最优萃取条件

Abstract:

To establish the appropriate extraction conditions for the analysis of volatile constituents from Feijoa sellowiana leaves by headspace solid phase micro-extraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS), the extraction conditions were investigated by orthogonal tests. The sum of the peak areas and total peak numbers of the volatile constituents were used as indices to optimize the extraction conditions by using L9(34) orthogonal array design. The results showed that the influence of temperature was larger and that of the sample amount was smaller for total peak numbers and peak areas. The optimum HS-SPME parameters were selected as follows: 0.6 g sample, extracted for 50 min at 50 ℃, and then desorbed for 6 min. Under these conditions, a total of 88 substances were detected. These substances can reflect the fragrant chemical components of Feijoa sellowiana leaves, and provide some references for the development and utilization of the leaves.

Key words: Feijoa sellowiana leaves, appropriate extraction condition, gas chromatography-mass spectrometry (GC-MS), headspace solid phase micro-extraction(HS-SPME), orthogonal test, volatile flavor

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