液相色谱法同时测定维药蜀葵花中芦丁、槲皮素和山柰酚

doi:10.3724/SP.J.1123.2015.05008

色谱 ›› 2015, Vol. 33 ›› Issue (12): 1269-1273.DOI: 10.3724/SP.J.1123.2015.05008

液相色谱法同时测定维药蜀葵花中芦丁、槲皮素和山柰酚

木合塔尔·吐尔洪^5,1,2, 热萨莱提·伊敏^5,1, 楚刚辉^1,2, 尹学博^1,2, 木尼热·阿不都克里木^5,2

1. 喀什大学化学与环境科学学院, 新疆喀什 844006;
2. 新疆特色药食用植物资源化学实验室, 新疆喀什 844006

收稿日期:2015-05-08 出版日期:2015-12-08 发布日期:2012-06-21
通讯作者: 木尼热·阿不都克里木
基金资助:
新疆维吾尔自治区高校科研计划重点课题项目(XJEDU2011147).

Determination of rutin, quercetin and kaempferol in althaea rosea (L) Gavan for Uyghur medicine by high performance liquid chromatography

MUHETAER·Tu'erhong^5,1,2, RESALAT·Yimin^5,1, CHU Ganghui^1,2, YIN Xuebo^1,2, MUNIRA·Abudukeremu^5,2

1. College of Chemistry and Environmental Sciences, Kashgar University, Kashgar 844006, China;
2. Xinjiang Laboratory of Native Medicinal and Edible Plant Resources Chemistry, Kashgar 844006, China

Received:2015-05-08 Online:2015-12-08 Published:2012-06-21

摘要/Abstract

摘要：

维药是祖国医药学不可分割的组成部分。维药现代化,即利用现代技术研究维药的有效成分,是维药科学化、标准化、规范化、商品化和产业化的必经之路。本文建立了维药蜀葵花中有效成分芦丁、槲皮素和山柰酚的选择性提取方法,优化了高效液相色谱法(HPLC)同时测定这3种有效成分的分析条件。采用HC-C₁₈色谱柱(250 mm×4.6 mm, 5 μm)和甲醇-0.4%磷酸(50:50, v/v)流动相,在柱温30 ℃和流速1.00 mL/min的条件下实现了3种物质之间以及和干扰物之间的基线分离。维药蜀葵花中芦丁、槲皮素及山柰酚的线性范围分别为12.5~150 μg/mL (r=0.9998), 12.5~125 μg/mL (r=0.9999)及12.5~125 μg/mL (r=0.9988),加标回收率(n=5)分别为100.3%(RSD=1.1%)、97.60%(RSD=0.47%)、97.75%(RSD=0.71%)。该方法实现了同时测定维药蜀葵花中芦丁、槲皮素及山柰酚,为其他黄酮类物质的开发应用提供了科学依据,同时也可为其他维药分析提供借鉴。

关键词: 高效液相色谱法, 槲皮素, 芦丁, 山柰酚, 蜀葵花

Abstract:

Uyghur medicine is one important part of the national medicine system. Uyghur medicine modernization, namely the study of effective components with modern technologies, is the only way for the scientification, standardization, and industrialization of Uyghur medicine. Here we developed a selective extraction method for rutin, quercetin and kaempferol in Althaea rosea (L) Gavan. The three active species were determined by high performance liquid chromatography (HPLC) with HC-C18 column (250 mm×4.6 mm, 5 μm) and the mobile phase of CH₃OH-0.4% H₃PO₄(50:50, v/v). Rutin, quercetin and kaempferol were baseline separated with each other and the interference species with flow rate of 1.0 mL/min and column temperature of 30 ℃. Under the optimal conditions, linear correlation were obtained in the mass concentration range of 12.5-150 μg/mL (r=0.9998) for rutin, 12.5-125 μg/mL (r=0.9999) for quercetin, and 12.5-125 μg/mL (r=0.9988) for kaempferol. The recoveries (n=5) of rutin, quercetin and kaempferol were 100.25% (RSD=1.1%), 97.60% (RSD=0.47%) and 97.75% (RSD=0.71%), respectively. The method can be used to determine the contents of rutin, quercetin and kaempferol in Althaea rosea (L) Gavan and provide the guidance for the analysis of the flavonoids in other Uyghur medicines.

Key words: Althaea rosea (L) Gavan, high performance liquid chromatography (HPLC), kaempferol, quercetin, rutin

中图分类号:

O658

木合塔尔·吐尔洪, 热萨莱提·伊敏, 楚刚辉, 尹学博, 木尼热·阿不都克里木. 液相色谱法同时测定维药蜀葵花中芦丁、槲皮素和山柰酚[J]. 色谱, 2015, 33(12): 1269-1273.

MUHETAER·Tu'erhong, RESALAT·Yimin, CHU Ganghui, YIN Xuebo, MUNIRA·Abudukeremu. Determination of rutin, quercetin and kaempferol in althaea rosea (L) Gavan for Uyghur medicine by high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2015, 33(12): 1269-1273.

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液相色谱法同时测定维药蜀葵花中芦丁、槲皮素和山柰酚

Determination of rutin, quercetin and kaempferol in althaea rosea (L) Gavan for Uyghur medicine by high performance liquid chromatography

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