色谱

色谱 ›› 2016, Vol. 34 ›› Issue (2): 146-151.DOI: 10.3724/SP.J.1123.2015.08021

• 研究论文 • 上一篇    下一篇

重力场流分离系统中载液及流速条件对分离的影响

郭爽1, 朱尘琪1, 高杨亚雅1, 邱百灵1, 吴迪1, 梁启慧1, 何嘉媛2, 韩南银1   

  1. 1. 北京大学药学院, 北京 100191;
    2. 中国农业大学, 北京 100193
  • 收稿日期:2015-08-19 出版日期:2016-02-08 发布日期:2012-09-11
  • 通讯作者: 韩南银

Effects of carrier liquid and flow rate on the separation in gravitational field-flow fractionation

GUO Shuang1, ZHU Chenqi1, YANG Yaya1, QIU Bailing1, WU Di1, LIANG Qihui1, HE Jiayuan2, HAN Nanyin1   

  1. 1. School of Pharmaceutical Sciences, Peking University, Beijing 100191, China;
    2. China Agricultural University, Beijing 100193, China
  • Received:2015-08-19 Online:2016-02-08 Published:2012-09-11

PDF

119

被引次数

6

摘要：

重力场流分离作为最简单的一种场流分离技术,常用于分离微米级颗粒。选择两种不同粒径(20 μ m和6 μ m)的聚苯乙烯(PS)颗粒作为样品,通过改变载液中叠氮化钠浓度、混合表面活性剂的比例及载液流速,利用自行设计生产的重力场流分离(gravitational flow field-flow fractionation, GrFFF)仪器,对颗粒混合样品进行分离,得到了相关谱图与数据,考察了这3种因素对分离效果(保留比(R)、塔板高度(H))的影响。结果表明:20 μ m PS颗粒的R值均大于6 μ m PS颗粒的R值,H值均小于6 μ m颗粒的H值;PS颗粒的R值与H值均随着载液中叠氮化钠浓度的增加而增加;但随着载液流速的增加,R值增加,H值减小。该研究为GrFFF系统的开发及应用提供了重要的参考价值。

关键词: 保留比, 场流分离系统, 聚苯乙烯颗粒, 流速, 塔板高度, 载液, 重力场流分离

Abstract:

Gravitational field-flow fractionation is the simplest field-flow fractionation technique in terms of principle and operation. The earth's gravity is its external field. Different sized particles are injected into a thin channel and carried by carrier fluid. The different velocities of the carrier liquid in different places results in a size-based separation. A gravitational field-flow fractionation (GrFFF) instrument was designed and constructed. Two kinds of polystyrene (PS) particles with different sizes (20 μ m and 6 μ m) were chosen as model particles. In this work, the separation of the sample was achieved by changing the concentration of NaN3, the percentage of mixed surfactant in the carrier liquid and the flow rate of carrier liquid. Six levels were set for each factor. The effects of these three factors on the retention ratio (R) and plate height (H) of the PS particles were investigated. It was found that R increased and H decreased with increasing particle size. On the other hand, the R and H increased with increasing flow rate. The R and H also increased with increasing NaN3 concentration. The reason was that the electrostatic repulsive force between the particles and the glass channel wall increased. The force allowed the samples approach closer to the channel wall. The results showed that the resolution and retention time can be improved by adjusting the experimental conditions. These results can provide important values to the further applications of GrFFF technique.

Key words: carrier liquid, field-flow fractionation (FFF), flow rate, gravitational field-flow fractionation (GrFFF), plate height, polystyrene (PS) particles, retention ratio

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