色谱

色谱 ›› 2016, Vol. 34 ›› Issue (8): 783-787.DOI: 10.3724/SP.J.1123.2016.03008

• 研究论文 • 上一篇    下一篇

高效液相色谱法研究白藜芦醇光致异构化动力学与平衡常数

李瑶, 陈静雅, 吴玉兰, 韩疏影, 郁红礼, 吴皓, 池玉梅   

  1. 南京中医药大学, 江苏 南京 210023
  • 收稿日期:2016-03-06 出版日期:2016-08-08 发布日期:2016-07-26
  • 通讯作者: 吴皓, 池玉梅
  • 基金资助:

    江苏高校优势学科建设工程项目（PAPD）.

Determination of the kinetics of photo-isomerization and equilibrium constant of resveratrol by high performance liquid chromatography

LI Yao, CHEN Jingya, WU Yulan, HAN Shuying, YU Hongli, WU Hao, CHI Yumei   

  1. Nanjing University of Traditional Chinese Medicine, Nanjing 210023, China
  • Received:2016-03-06 Online:2016-08-08 Published:2016-07-26
  • Supported by:

    Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

PDF

85

被引次数

1

摘要：

反式白藜芦醇有多种生理活性，自然资源丰富，但存在光不稳定现象。以光致异构平衡对照品溶液，采用高效液相色谱建立了同时测定顺、反异构体浓度的方法。方法学考察显示符合含量测定要求，反式异构体在1.5~60 μ mol/L、顺式异构体在1.5~55 μ mol/L范围内线性关系良好，R2均达0.999。将该方法应用于研究光致异构化动力学及平衡常数，结果显示反式白藜芦醇在室温避光条件下中性、偏酸性及乙醇介质中稳定；25 ℃时在50%（v/v）乙醇介质中的光异构平衡常数为11.7±0.3。以光致异构平衡液制作标准曲线的策略简单方便，具有可操作性；反式白藜芦醇的稳定性与介质有关，在pH < 7的溶液中光致异构化为一级反应。

关键词: 白藜芦醇, 动力学, 高效液相色谱, 光致异构化, 平衡常数

Abstract:

Trans-resveratrol (C14H12O3, 5-(4-hydroxystyryl)benzene-1, 3-diol), with a variety of physiological activities and abundant sources, shows instability to light irradiation. The control solution was balanced by photo-isomerization and high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of trans-resveratrol and cis-resveratrol. The methodology study showed good linear correlations (R2=0.999) in the range of 1.5-60 μ mol/L of trans-resveratrol and 1.5-55 μ mol/L of cis-resveratrol which conforms to the request of content determination. The method was used to study the kinetics and equilibrium constant of photo-isomerization. The results showed that trans-resveratrol protected from light was stable in neutral, weak acid and ethanol media at room temperature. The photo-isomerization equilibrium constant was 11.7±0.3 at 25 ℃ in dilute ethanol solution. The method of making the standard curve by photo-isomerization equilibrium solution is simple, convenient and practicable. The stability of trans-resveratrol is related to the medium. When pH value is less than 7, the photo-isomerization is a first order reaction.

Key words: equilibrium constant, high performance liquid chromatography (HPLC), kinetics, photo-isomerization, resveratrol

中图分类号: