色谱

色谱 ›› 2017, Vol. 35 ›› Issue (9): 1014-1021.DOI: 10.3724/SP.J.1123.2017.04030

• 研究论文 • 上一篇    

大孔树脂-高速逆流色谱法分离纯化地黄中毛蕊花糖苷

步知思, 何青, 赵如诗, 楚楚, 李行诺, 童胜强   

  1. 浙江工业大学药学院, 浙江 杭州 310032
  • 收稿日期:2017-04-24 出版日期:2017-09-08 发布日期:2017-09-15
  • 通讯作者: 童胜强,Tel/Fax:+86 571 88320984,E-mail:sqtong@zjut.edu.cn.

Separation and purification of acteoside from Rehmannia glutinosa by combining macroporous resin with high-speed countercurrent chromatography

BU Zhisi, HE Qing, ZHAO Rushi, CHU Chu, LI Xingnuo, TONG Shengqiang   

  1. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China
  • Received:2017-04-24 Online:2017-09-08 Published:2017-09-15

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摘要：

该文建立了大孔树脂-高速逆流色谱分离中药材地黄中有效成分毛蕊花糖苷的方法。考察了4种大孔树脂对地黄粗提物中毛蕊花糖苷的静态吸附与解吸情况，其中D101大孔树脂对目标成分的吸附率与解吸率最理想，实验结果表明体积分数为10%的乙醇洗脱得到的毛蕊花糖苷含量最高，目标成分含量从4.9%提高到32.6%。最后，部分纯化的样品（165 mg）采用高速逆流色谱进一步纯化，两相溶剂系统由乙酸乙酯-正丁醇-水（1：4：5，v/v/v）组成，分离得到45 mg纯度为96%的毛蕊花糖苷。

关键词: 大孔树脂, 地黄, 毛蕊花糖苷, 逆流色谱

Abstract:

Acteoside,a chemical component of the traditional Chinese medicinal herb Rehmannia glutinosa,was separated from the plant by a combination of macroporous resin column chromatography and high-speed countercurrent chromatography.The static adsorption and desorption of acteoside in the crude extract were in-vestigated using four kinds of macroporous resin,among which the D101 macroporous resin presented the best adsorption and desorption rates toward the compound.The crude extract was then gradient-eluted with increa-sing volume percentages of ethanol,where it was found that the highest content of acteoside was obtained when using 10%(v/v) ethanol eluent,in which the purity of acteoside was increased from 4.9% to 32.6%.Then,the partially purified crude extract (165 mg) was further purified by high-speed countercurrent chroma-tography using a two-phase solvent system consisting of ethyl acetate-n-butanol-water (1:4:5,v/v/v),yielding 45 mg of acteoside with 96% purity.

Key words: Rehmannia glutinosa, acteoside, countercurrent chromatography, macroporous resin

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