液相色谱-同位素比质谱技术发展及应用研究进展

doi:10.3724/SP.J.1123.2019.12007

摘要/Abstract

摘要：

液相色谱-同位素比质谱（LC-IRMS）是一种特征化合物同位素分析技术，该技术利用LC IsoLink接口设备实现液相色谱与同位素比质谱的联用，通过检测目标物质的稳定碳同位素比（δ¹³C），实现样品的产地来源与品质真实性鉴定。该文总结了IRMS与LC-IRMS技术的概况，以及过去20年LC-IRMS的发展历程；归纳整理了LC-IRMS在食品安全、生态与环境、生命科学及考古学等领域的应用情况；评述了LC-IRMS面临的技术局限、挑战及其未来的发展趋势。

关键词: 同位素比质谱, 液相色谱, 特征化合物同位素分析, δ¹³C, 综述

Abstract:

The first commercial interface for hyphenating liquid chromatography with isotope ratio mass spectrometry (LC-IRMS) was not available until 2004, and it was commercialized under the name LC IsoLink by Thermo Finnigan. LC-IRMS, a method of compound-specific isotope analysis, has enabled the detection of stable carbon isotope ratios (δ¹³C) in targeted substances in order to determine their origin and authenticity. This paper provides an overview of IRMS and LC-IRMS techniques, the history and detailed classification of the latter over the past twenty years. The application of LC-IRMS to the fields of food safety, environment and ecology, life sciences, and archeology are reviewed. Finally, the technical limitations, current challenges, and future development trends of LC-IRMS are outlined.

Key words: isotope ratio mass spectrometry (IRMS), liquid chromatography (LC), compound-specific isotope analysis, δ¹³C, review

丁博, 谢建军, 王志元, 陶移文, 黄洪波, 陈文锐, 姚孝宝. 液相色谱-同位素比质谱技术发展及应用研究进展[J]. 色谱, 2020, 38(6): 627-638.

DING Bo, XIE Jianjun, WANG Zhiyuan, TAO Yiwen, HUANG Hongbo, CHEN Wenrui, YAO Xiaobao. Advances in development and application of liquid chromatography coupled with isotope ratio mass spectrometry[J]. Chinese Journal of Chromatography, 2020, 38(6): 627-638.

图/表 5

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Chromatographic method	Column	Analytes	Field	Ref.
Ion interaction	Dr. Maisch Repromer Ca	sophisticated honey	food quality	^[36]
Mixed mode column	Primesep A	amino acid δ¹³C of keratin from single hairs	archaeology	^[37]
Ion interaction	Alltech 700 CH cation exchange	δ¹³C of dissolved inorganic carbon	environmental science	^[38]
Direct injection	no column	δ¹³C of dissolved inorganic carbon	environmental science	^[39]
Reversed phase column	X-Bridge C18	sulfonamide antibiotic sulfamethoxazole	environmental science	^[40]
Ion interaction	Hyper REZ XP Carbohydrate Ca²⁺	honey	food quality	^[41]
Reversed phase column	YMC-Triart C18	halogenated benzoic acids	environmental science	^[42]
Ion interaction	Sugar-Pak I analytical column	honey	food quality	^[43]
Ion interaction	Reprosil Repromer H column	xylitol in chewing gum	food quality	^[44]
Mixed mode column	Primesep A	amino acid δ¹³C of tendon collagen and hair keratin	archaeology	^[45]
Ion interaction	Sugar-Pak analytical column	organic acids in commercial honey	food quality	^[46]
Reversed phase column	Waters XBridge C18	caffeine, vanillin, histidine, glucose, phenyalanine	food quality	^[47]
Reversed phase column	Atlantis T3	metabolites of herbicides	environmental science	^[48]
Ion interaction	Phenomenex Rezek RCM Ca²⁺	chinese acacia honey	food quality	^[49]
Reversed phase column	Atlantis T3 column	desphenylchloridazon of pesticides	environmental science	^[50]

Chromatographic method	Column	Analytes	Field	Ref.
Ion interaction	Dr. Maisch Repromer Ca	sophisticated honey	food quality	^[36]
Mixed mode column	Primesep A	amino acid δ¹³C of keratin from single hairs	archaeology	^[37]
Ion interaction	Alltech 700 CH cation exchange	δ¹³C of dissolved inorganic carbon	environmental science	^[38]
Direct injection	no column	δ¹³C of dissolved inorganic carbon	environmental science	^[39]
Reversed phase column	X-Bridge C18	sulfonamide antibiotic sulfamethoxazole	environmental science	^[40]
Ion interaction	Hyper REZ XP Carbohydrate Ca²⁺	honey	food quality	^[41]
Reversed phase column	YMC-Triart C18	halogenated benzoic acids	environmental science	^[42]
Ion interaction	Sugar-Pak I analytical column	honey	food quality	^[43]
Ion interaction	Reprosil Repromer H column	xylitol in chewing gum	food quality	^[44]
Mixed mode column	Primesep A	amino acid δ¹³C of tendon collagen and hair keratin	archaeology	^[45]
Ion interaction	Sugar-Pak analytical column	organic acids in commercial honey	food quality	^[46]
Reversed phase column	Waters XBridge C18	caffeine, vanillin, histidine, glucose, phenyalanine	food quality	^[47]
Reversed phase column	Atlantis T3	metabolites of herbicides	environmental science	^[48]
Ion interaction	Phenomenex Rezek RCM Ca²⁺	chinese acacia honey	food quality	^[49]
Reversed phase column	Atlantis T3 column	desphenylchloridazon of pesticides	environmental science	^[50]

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