色谱

色谱

• 专论综述 • 上一篇    下一篇

嗜硫色谱纯化抗体技术的研究进展

刘玄,宋宏新

  

  1. Life Science & Engineering College, Shaanxi University of Science &Technology, Xianyang 712081, China
  • 收稿日期:2004-12-15 修回日期:1900-01-01 出版日期:2006-01-30 发布日期:1987-09-25
  • 通讯作者: 刘玄

Development and Application of Thiophilic Adsorption Chromatography for Antibody Purification

LIU Xuan, SONG Hongxin   

  1. Life Science & Engineering College, Shaanxi University of Science &Technology, Xianyang 712081, China
  • Received:2004-12-15 Revised:1900-01-01 Online:2006-01-30 Published:1987-09-25

PDF

674

摘要：

嗜硫色谱在高盐环境下对抗体及其他某些蛋白质产生特异性吸附,再在低盐条件下洗脱,可获得高纯度及高回收率的蛋白质产品,是一种新型蛋白质纯化技术。该文对嗜硫色谱及其在抗体纯化中的应用做了综述。

关键词: 抗体纯化 , 色谱, 嗜硫吸附

Abstract:

Thiophilic adsorption chromatography binds antibodies and other proteins in the presence of high concentration of salt. When eluted by decreasing the salt concentration, the high purity and recovery of proteins can be achieved. It is a new chromatographic technology for the purification of proteins. The review introduces the method for the purification of antibodies and covers the development and application of the original technology.

Key words: antibody purification , chromatography, thiophilic adsorption