色谱

色谱

• 专论综述 • 上一篇    下一篇

生物样品中短链脂肪酸的提取与测定

谭力1,2,鞠熀先1,黎介寿2

  

  1. 1.Key Laboratory of Analytical Chemistry for Life Science， Education Ministry of China, Nanjing University, Nanjing 210093, China; 2.Research Institute of General Surgery, Nanjing General Hospital of Nanjing Command, Nanjing 210002, China
  • 收稿日期:2004-11-29 修回日期:1900-01-01 出版日期:2006-01-30 发布日期:1987-09-25
  • 通讯作者: 谭力

Extraction and Determination of Short-Chain Fatty Acids in Biological Samples

TAN Li1,2, JU Huangxian1, LI Jieshou2   

  1. 1.Key Laboratory of Analytical Chemistry for Life Science， Education Ministry of China, Nanjing University, Nanjing 210093, China; 2.Research Institute of General Surgery, Nanjing General Hospital of Nanjing Command, Nanjing 210002, China
  • Received:2004-11-29 Revised:1900-01-01 Online:2006-01-30 Published:1987-09-25

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摘要：

综述了生物样品(主要为粪便、尿液、血液和培养液)中短链脂肪酸的提取与测定方法，讨论了各种样品提取方法的优缺点，并比较了气相色谱、高效液相色谱和毛细管电泳分离检测的优点和局限性。引用文献63篇。

关键词: 短链脂肪酸, 高效液相色谱, 毛细管电泳, 气相色谱, 生物样品, 提取方法, 综述

Abstract:

Short-chain fatty acids are organic acids with 1-6 carbon atoms. Their physiological functions and clinical applications have attracted considerable attention. Their high polarity, low ultraviolet (UV) absorbance, low contents in biological samples, high volatility, good water-solubility and easy adsorption on metal and glass surfaces result in some difficulties in their separation and determination. This review focuses on the methods for extraction of short-chain fatty acids in biological samples such as feces, urine, blood and culture solutions by distillation, high speed centrifugation, liquid-solid extraction, solid phase microextration, supercritical fluid extraction and liquid phase microextraction and determination by gas chromatography, high performance liquid chromatography and capillary electrophoresis. Sixty-three references are cited.

Key words: review , biological materials, capillary electrophoresis (CE), extraction method, gas chromatography (GC), high performance liquid chromatography (HPLC), short-chain fatty acids (SCFA)