色谱

色谱

• 研究简报 • 上一篇    下一篇

酪蛋白酶解反应复杂产物的色谱定量分析

于艳军,齐崴,何志敏   

  1. Enzyme Technology Laboratory, Chemical Engineering Research Center, Tianjin University, Tianjin 300072, China
  • 收稿日期:2004-03-26 修回日期:1900-01-01 出版日期:2005-03-30 发布日期:1988-12-25
  • 通讯作者: 何志敏
  • 基金资助:

Quantitative Analysis of Complicated Casein Hydrolysates by High Performance Size Exclusion Chromatography

YU Yanjun, QI Wei, HE Zhimin   

  1. Enzyme Technology Laboratory, Chemical Engineering Research Center, Tianjin University, Tianjin 300072, China
  • Received:2004-03-26 Revised:1900-01-01 Online:2005-03-30 Published:1988-12-25

PDF

667

被引次数

3

摘要： 根据酪蛋白酶解产物的高效凝胶排阻色谱(HPSEC)定性分析结果,选取不同截留相对分子质量的超滤膜对酶解产物进行截分,制得3种相对分子质量分布相对集中的区间组分;建立各组分峰面积与质量浓度之间的线性关系,由此定量计算不同反应时间的酶解产物中相应相对分子质量区间组分的质量浓度。研究表明,将膜分离方法与色谱分析相结合可实现对蛋白质、多肽等复杂体系相对精确的定量分析。

关键词: 定量分析 , 复杂体系, 高效凝胶排阻色谱, 酪蛋白, 酶促水解, 膜分离

Abstract: The enzymatic hydrolysates of casein are so complicated that there has no effective method for quantitative analysis. On the basis of membrane separation and high performance size exclusion chromatography (HPSEC), standard peptides with different relative molecular mass ranges were prepared, and the linear relationships between mass concentration of the standard peptides and the corresponding peak areas were established. Consequently, mass concentration of the different hydrolysates at different reaction time can be accurately calculated. The combination of chromatography and membrane separation is of great importance to the quantitative analysis of the complicated hydrolysates, which can be also applied to the other macromolecular systems, such as carbohydrates.

Key words: complicated system, enzymatic hydrolysis, high performance size exclusion chromatography, membrane separation, quantitative analysis , casein