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色谱

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毛细管电泳-安培检测法用于7-甲基鸟苷与丝裂霉素C分离检测的研究

张兰,何聿,陈毅挺,童萍,陈国南   

  1. School of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China
  • 收稿日期:2004-11-19 修回日期:1900-01-01 出版日期:2005-03-30 发布日期:1988-12-25
  • 通讯作者: 张兰
  • 基金资助:
    福建省科技厅科技攻关重点基金资助项目(2004Y003)和福州大学科技发展基金资助项目(2004-XQ-08).

Study on the Simultaneous Determination of 7-Methylguanosine and Mitomycin C by Capillary Electrophoresis with Amperometric Detection

ZHANG Lan, HE Yu, CHEN Yiting, TONG Ping, CHEN Guonan   

  1. School of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China
  • Received:2004-11-19 Revised:1900-01-01 Online:2005-03-30 Published:1988-12-25

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摘要： 建立了一种同时分离检测7-甲基鸟苷与丝裂霉素C的毛细管电泳-安培检测方法。在950 mV电极(工作电极:0.3 mm微型石墨圆盘电极;参比电极:Ag/AgCl;辅助电极:Pt丝)电位下,于20 mmol/L的磷酸盐缓冲体系(pH 9.4)中,采用18 kV的分离电压进行分离。在最佳条件下,7-甲基鸟苷与丝裂霉素C在10 min内实现分离,7-甲基鸟苷与丝裂霉素C的线性范围均为0.50~50 mg/L,检测限分别为0.050 mg/L与0.025 mg/L。将该方法用于模拟尿样和模拟兔血清样的检测,7-甲基鸟苷与丝裂霉素C的回收率为93.0%～97.2%,结果令人满意。

关键词: 7-甲基鸟苷, 安培检测法, 毛细管电泳, 丝裂霉素C

Abstract: A method based on capillary electrophoresis with amperometric detection (CE-AD) has been developed for the simultaneous determination of 7-methylguanosine and mitomycin C. The detection electrode was a 0.3 mm carbon disc electrode at a working potential of 950 mV (versus Ag/AgCl electrode). A suitable electrolyte consists of 20 mmol/L phosphate buffer (pH 9.4), with the separation voltage of 18 kV, the injection time of 10 s, and the injection voltage of 18 kV. Under optimum conditions, the two analytes could be well separated within 10 min in a 60 cm length capillary. The current responses were linear over about two orders of magnitude (0.50-50 mg/L) with detection limits (S/N=3) of 0.050 and 0.025 mg/L for 7-methylguanosine and mitomycin C, respectively. The proposed method was successfully applied to determine the synthetic urine and serum samples. The recoveries were found in the range of 93.0%-97.2%.

Key words: 7-methylguanosine, amperometric detection, mitomycin , capillary electrophoresis