色谱

色谱

• 专论与综述 • 上一篇    下一篇

以亚临界水为流动相的高效液相色谱方法的进展

苏奕菘1,郑政峰1,张维冰1,2   

  1. 1. Department of Chemistry, National Chung-Hsing University, Taichung 402, China; 2. National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China
  • 收稿日期:2004-10-11 修回日期:1900-01-01 出版日期:2005-05-30 发布日期:1988-09-25
  • 通讯作者: 张维冰
  • 基金资助:

The Method Development of Subcritical Water Chromatography

SU Yisong1, JEN Jen-Fon1, ZHANG Weibing1,2   

  1. 1. Department of Chemistry, National Chung-Hsing University, Taichung 402, China; 2. National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China
  • Received:2004-10-11 Revised:1900-01-01 Online:2005-05-30 Published:1988-09-25

PDF

637

被引次数

26

摘要： 由于纯水在高温高压下形成亚临界液体水时氢键网络发生改变,导致其极性、粘度等物理性质产生较大的变化。以亚临界水为流动相的高效液相色谱方法(SubWC)是近年来发展起来的一种新型分离技术。SubWC的仪器系统可以采用通过改装的一般的气相色谱(GC)或液相色谱(LC)装置;分离色谱柱既可以采用液相色谱填充柱,也可以采用类似于GC的毛细管柱;选择性既可以通过调节柱系统的温度和压力,也可以通过在流动相中添加有机调节剂或盐类进行调节;检测既可用氢火焰离子化检测器检测,也可用紫外检测器检测,极大地拓宽了色谱分离和最佳条件选择的范围。SubWC无论在仪器系统、流动相的洗脱还是固定相的选择等方面均有一定的特征。这种新的分离模式目前尚处于研究与开发阶段,且多用于极性、中等极性样品的快速分离。

关键词: 固定相, 流动相, 亚临界水, 液相色谱, 综述

Abstract: Subcritical water is formed from pure water under high temperature and high pressure. The change of hydrogen-bond network results in the obvious change of physical properties of water such as polarity and viscosity. Subcritical water chromatography (SubWC), as a method of high performance liquid chromatography which takes subcritical water as mobile phase, is one kind of novel separation technique developed recently. Common gas chromatographic or liquid chromatographic instrument modified properly can be used as the instrument for SubWC. Both packed columns used in liquid chromatography and capillary columns used in gas chromatography can be applied in SubWC. The selectivity can be adjusted by changing column temperature or pressure and addition of the organic modifiers or salts in mobile phase. Both flame ionization detector and ultraviolet detector can be used as the detecting part of SubWC. Thus the range of its application and selection of optimal conditions are largely broadened. Currently this novel separation mode is still in the stage of research and development and mostly used in the fast separation of samples with polar and medium polar. The characteristics of SubWC are illustrated from the aspects and design of instruments, the elution characteristics of mobile phase and the selection of stationary phase. Moreover, the recent development of SubWC is systematically reviewed.

Key words: mobile phase, review , stationary phase, subcritical water, liquid chromatography